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dc.contributor.authorAlghamdi, Rana A
dc.contributor.authorExposito-Rodriguez, Marino
dc.contributor.authorMullineaux, Philip M
dc.contributor.authorBrooke, Greg N
dc.contributor.authorLaissue, Philippe P
dc.date.accessioned2022-01-07T16:52:37Z
dc.date.available2022-01-07T16:52:37Z
dc.date.issued2021
dc.date.submitted2021-07-09
dc.identifier.issn2296-634X
dc.identifier.other738786
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332414
dc.description.abstractPhototoxicity is a significant constraint for live cell fluorescence microscopy. Excessive excitation light intensities change the homeostasis of the observed cells. Erroneous and misleading conclusions may be the problematic consequence of observing such light-induced pathophysiology. In this study, we assess the effect of blue light, as commonly used for GFP and YFP excitation, on a motile mammalian cell line. Tracking PC3 cells at different light doses and intensities, we show how motility can be used to reliably assess subtle positive and negative effects of illumination. We further show that the effects are a factor of intensity rather than light dose. Mitotic delay was not a sensitive indicator of phototoxicity. For early detection of the effect of blue light, we analysed the expression of genes involved in oxidative stress. This study addresses the need for relatively simple and sensitive methods to establish a dose-response curve for phototoxicity in mammalian cell line models. We conclude with a working model for phototoxicity and recommendations for its assessment.
dc.languageen
dc.publisherFrontiers Media SA
dc.subjectCell and Developmental Biology
dc.subjectfluorescence
dc.subjectmicroscopy—light
dc.subjectlive imaging
dc.subjectreactive oxygen species (ROS)
dc.subjectlight intensity (irradiance)
dc.titleAssessing Phototoxicity in a Mammalian Cell Line: How Low Levels of Blue Light Affect Motility in PC3 Cells.
dc.typeArticle
dc.date.updated2022-01-07T16:52:36Z
prism.publicationNameFront Cell Dev Biol
prism.volume9
dc.identifier.doi10.17863/CAM.79860
dcterms.dateAccepted2021-11-16
rioxxterms.versionofrecord10.3389/fcell.2021.738786
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.identifier.eissn2296-634X
cam.issuedOnline2021-12-17


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