Small molecule electro-optical binding assay using nanopores.
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Publication Date
2019-04-17Journal Title
Nat Commun
ISSN
2041-1723
Publisher
Springer Science and Business Media LLC
Volume
10
Issue
1
Number
ARTN 1797
Pages
1797
Type
Article
This Version
VoR
Physical Medium
Electronic
Metadata
Show full item recordCitation
Cai, S., Sze, J. Y., Ivanov, A. P., & Edel, J. B. (2019). Small molecule electro-optical binding assay using nanopores.. Nat Commun, 10 (1. ARTN 1797), 1797. https://doi.org/10.1038/s41467-019-09476-4
Abstract
The identification of short nucleic acids and proteins at the single molecule level is a major driving force for the development of novel detection strategies. Nanopore sensing has been gaining in prominence due to its label-free operation and single molecule sensitivity. However, it remains challenging to detect small molecules selectively. Here we propose to combine the electrical sensing modality of a nanopore with fluorescence-based detection. Selectivity is achieved by grafting either molecular beacons, complementary DNA, or proteins to a DNA molecular carrier. We show that the fraction of synchronised events between the electrical and optical channels, can be used to perform single molecule binding assays without the need to directly label the analyte. Such a strategy can be used to detect targets in complex biological fluids such as human serum and urine. Future optimisation of this technology may enable novel assays for quantitative protein detection as well as gene mutation analysis with applications in next-generation clinical sample analysis.
Keywords
Biosensing Techniques, DNA, Fluorescence, Humans, Nanopores, Nanotechnology, Proteins
Identifiers
External DOI: https://doi.org/10.1038/s41467-019-09476-4
This record's URL: https://www.repository.cam.ac.uk/handle/1810/332701
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