Single-cell mapping of DNA G-quadruplex structures in human cancer cells.
Publication Date
2021-12-08Journal Title
Sci Rep
ISSN
2045-2322
Publisher
Springer Science and Business Media LLC
Volume
11
Issue
1
Language
en
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Hui, W. W., Simeone, A., Zyner, K. G., Tannahill, D., & Balasubramanian, S. (2021). Single-cell mapping of DNA G-quadruplex structures in human cancer cells.. Sci Rep, 11 (1) https://doi.org/10.1038/s41598-021-02943-3
Description
Funder: Herchel Smith Funds
Abstract
G-quadruplexes (G4s) are four-stranded DNA secondary structures that form in guanine-rich regions of the genome. G4s have important roles in transcription and replication and have been implicated in genome instability and cancer. Thus far most work has profiled the G4 landscape in an ensemble of cell populations, therefore it is critical to explore the structure-function relationship of G4s in individual cells to enable detailed mechanistic insights into G4 function. With standard ChIP-seq methods it has not been possible to determine if G4 formation at a given genomic locus is variable between individual cells across a population. For the first time, we demonstrate the mapping of a DNA secondary structure at single-cell resolution. We have adapted single-nuclei (sn) CUT&Tag to allow the detection of G4s in single cells of human cancer cell lines. With snG4-CUT&Tag, we can distinguish cellular identity from a mixed cell-type population solely based on G4 features within individual cells. Our methodology now enables genomic investigations on cell-to-cell variation of a DNA secondary structure that were previously not possible.
Keywords
Article, /631/92/147, /631/61/514/2254, /631/61/212, /631/1647, /631/208/176, article
Sponsorship
Cancer Research UK core grant (C9545/A19836) and Cancer Research UK programme grant (C9681/A29214). S.B. is a senior investigator of the Wellcome Trust (209441/Z/17/Z) and is funded by Herchel Smith Funds.
Funder references
Cancer Research UK (CB4330)
Wellcome Trust (209441/Z/17/Z)
Identifiers
s41598-021-02943-3, 2943
External DOI: https://doi.org/10.1038/s41598-021-02943-3
This record's URL: https://www.repository.cam.ac.uk/handle/1810/333011
Rights
Licence:
http://creativecommons.org/licenses/by/4.0/
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