FeII 4L4 tetrahedron binds and aggregates DNA G-quadruplexes.
Royal Society of Chemistry (RSC)
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Zhu, J., Yan, Z., Bošković, F., Haynes, C. J., Kieffer, M., Greenfield, J. L., Wang, J., et al. (2021). FeII 4L4 tetrahedron binds and aggregates DNA G-quadruplexes.. Chem Sci, 12 (43), 14564-14569. https://doi.org/10.1039/d1sc04430c
Since the discovery of the G-quadruplex (G4) structure in telomeres in 1980s, studies have established the role it plays in various biological processes. Here we report binding between DNA G4 and a self-assembled tetrahedral metal-organic cage 1 and consequent formation of aggregates, whereby the cage protects the DNA G4 from cleavage by S1 nuclease. We monitor DNA-cage interaction using fluorescence spectroscopy, firstly by quenching of a fluorescent label appended to the 5' end of G4. Secondly, we detect the decrease in fluorescence of the G4-selective dyes thioflavin-T and Zn-PPIX bound to various DNA G4 sequences following the addition of cage 1. Our results demonstrate that 1 interacts with a wide range of G4s. Moreover, gel electrophoresis, circular dichroism and dynamic light scattering measurements establish the binding of 1 to G4 and indicate the formation of aggregate structures. Finally, we find that DNA G4 contained in an aggregate of cage 1 is protected from cleavage by S1 nuclease.
Engineering and Physical Sciences Research Council (EP/M008258/1)
European Research Council (647144)
External DOI: https://doi.org/10.1039/d1sc04430c
This record's URL: https://www.repository.cam.ac.uk/handle/1810/333080
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/