FeII 4L4 tetrahedron binds and aggregates DNA G-quadruplexes.
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Authors
Publication Date
2021-11-10Journal Title
Chem Sci
ISSN
2041-6520
Publisher
Royal Society of Chemistry (RSC)
Volume
12
Issue
43
Pages
14564-14569
Language
eng
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Zhu, J., Yan, Z., Bošković, F., Haynes, C. J., Kieffer, M., Greenfield, J. L., Wang, J., et al. (2021). FeII 4L4 tetrahedron binds and aggregates DNA G-quadruplexes.. Chem Sci, 12 (43), 14564-14569. https://doi.org/10.1039/d1sc04430c
Abstract
Since the discovery of the G-quadruplex (G4) structure in telomeres in 1980s, studies have established the role it plays in various biological processes. Here we report binding between DNA G4 and a self-assembled tetrahedral metal-organic cage 1 and consequent formation of aggregates, whereby the cage protects the DNA G4 from cleavage by S1 nuclease. We monitor DNA-cage interaction using fluorescence spectroscopy, firstly by quenching of a fluorescent label appended to the 5' end of G4. Secondly, we detect the decrease in fluorescence of the G4-selective dyes thioflavin-T and Zn-PPIX bound to various DNA G4 sequences following the addition of cage 1. Our results demonstrate that 1 interacts with a wide range of G4s. Moreover, gel electrophoresis, circular dichroism and dynamic light scattering measurements establish the binding of 1 to G4 and indicate the formation of aggregate structures. Finally, we find that DNA G4 contained in an aggregate of cage 1 is protected from cleavage by S1 nuclease.
Sponsorship
Engineering and Physical Sciences Research Council (EP/M008258/1)
European Research Council (647144)
Identifiers
34881008, PMC8580047
External DOI: https://doi.org/10.1039/d1sc04430c
This record's URL: https://www.repository.cam.ac.uk/handle/1810/333080
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