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dc.contributor.authorJanssen, Anne
dc.contributor.authorBreusegem, Sophia
dc.contributor.authorLarrieu, Delphine
dc.date.accessioned2022-01-28T16:41:02Z
dc.date.available2022-01-28T16:41:02Z
dc.date.issued2022-01-20
dc.identifier.issn2073-4409
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/333231
dc.description.abstractAny given cell type has an associated "normal" nuclear morphology, which is important to maintain proper cellular functioning and safeguard genomic integrity. Deviations from this can be indicative of diseases such as cancer or premature aging syndrome. To accurately assess nuclear abnormalities, it is important to use quantitative measures of nuclear morphology. Here, we give an overview of several nuclear abnormalities, including micronuclei, nuclear envelope invaginations, blebs and ruptures, and review the current methods used for image-based quantification of these abnormalities. We discuss several parameters that can be used to quantify nuclear shape and compare their outputs using example images. In addition, we present new pipelines for quantitative analysis of nuclear blebs and invaginations. Quantitative analyses of nuclear aberrations and shape will be important in a wide range of applications, from assessments of cancer cell anomalies to studies of nucleus deformability under mechanical or other types of stress.
dc.description.sponsorshipFEBS Long-Term Fellowship.
dc.languageen
dc.publisherMDPI AG
dc.subjectnuclear envelope
dc.subjectnuclear envelope abnormality
dc.subjectlamina
dc.subjectnuclear morphology
dc.titleCurrent Methods and Pipelines for Image-Based Quantitation of Nuclear Shape and Nuclear Envelope Abnormalities.
dc.typeArticle
dc.date.updated2022-01-28T16:41:01Z
prism.issueIdentifier3
prism.publicationNameCells
prism.volume11
dc.identifier.doi10.17863/CAM.80654
dcterms.dateAccepted2022-01-18
rioxxterms.versionofrecord10.3390/cells11030347
rioxxterms.versionVoR
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidLarrieu, Delphine [0000-0002-2335-9361]
dc.identifier.eissn2073-4409
pubs.funder-project-idWellcome Trust (206242/Z/17/Z)
pubs.funder-project-idFederation of the European Biochemical Societies (FEBS) (Anne Janssen FEBS LTF)
cam.issuedOnline2022-01-20


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