Ozonation of Whole Blood Results in an Increased Release of Microparticles from Blood Cells.
Authors
Boczkowska-Radziwon, Barbara
Olbromski, Piotr Józef
Rogowska, Anna
Bujno, Magdalena
Żebrowska, Agnieszka
Średziński, Dariusz
Polityńska, Barbara
Radziwon, Piotr
Publication Date
2022-01-21Journal Title
Biomolecules
ISSN
2218-273X
Publisher
MDPI AG
Volume
12
Issue
2
Language
en
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Boczkowska-Radziwon, B., Olbromski, P. J., Rogowska, A., Bujno, M., Myśliwiec, M., Żebrowska, A., Średziński, D., et al. (2022). Ozonation of Whole Blood Results in an Increased Release of Microparticles from Blood Cells.. Biomolecules, 12 (2) https://doi.org/10.3390/biom12020164
Abstract
Autohemotherapy with ozonated blood is used in the treatment of a broad spectrum of clinical disorders. Ozone demonstrates strong oxidizing properties and causes damage to cell membranes. The impact of whole-blood ozonation on the release of microparticles from blood and endothelial cells and the concentration of selected markers in the hemostatic system (APTT, PT, D-dimer, fibrinogen) were investigated. Venous blood, obtained from 19 healthy men, was split into four equal parts and treated with air, 15 µg/mL ozone, or 30 µg/mL ozone, or left untreated. The number and types of microparticles released were determined using flow cytometry on the basis of surface antigen expression: erythrocyte-derived microparticles (CD235+), platelet-derived microparticles (CD42+), leukocyte-derived microparticles (CD45+), and endothelial-derived microparticles (CD144+). The study is the first to demonstrate that ozone induces a statistically significant increase in the number of microparticles derived from blood and endothelial cells. Although statistically significant, the changes in some coagulation factors were somewhat mild and did not exceed normal values.
Keywords
ozone, microparticles, autohemotherapy, blood coagulation
Sponsorship
Medical University of Białystok (N/ST/ZB/16/002/1152)
Identifiers
External DOI: https://doi.org/10.3390/biom12020164
This record's URL: https://www.repository.cam.ac.uk/handle/1810/333239
Rights
Licence:
https://creativecommons.org/licenses/by/4.0/
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