Carbon flux through photosynthesis and central carbon metabolism show distinct patterns between algae, C 3 and C 4 plants
Authors
Arrivault, Stéphanie
Ishihara, Hirofumi
Hoppe, Ines
Höhne, Melanie
Publication Date
2021-12-23Journal Title
Nature Plants
Publisher
Nature Publishing Group UK
Volume
8
Issue
1
Pages
78-91
Language
en
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Treves, H., Küken, A., Arrivault, S., Ishihara, H., Hoppe, I., Erban, A., Höhne, M., et al. (2021). Carbon flux through photosynthesis and central carbon metabolism show distinct patterns between algae, C 3 and C 4 plants. Nature Plants, 8 (1), 78-91. https://doi.org/10.1038/s41477-021-01042-5
Abstract
Abstract: Photosynthesis-related pathways are regarded as a promising avenue for crop improvement. Whilst empirical studies have shown that photosynthetic efficiency is higher in microalgae than in C3 or C4 crops, the underlying reasons remain unclear. Using a tailor-made microfluidics labelling system to supply 13CO2 at steady state, we investigated in vivo labelling kinetics in intermediates of the Calvin Benson cycle and sugar, starch, organic acid and amino acid synthesis pathways, and in protein and lipids, in Chlamydomonas reinhardtii, Chlorella sorokiniana and Chlorella ohadii, which is the fastest growing green alga on record. We estimated flux patterns in these algae and compared them with published and new data from C3 and C4 plants. Our analyses identify distinct flux patterns supporting faster growth in photosynthetic cells, with some of the algae exhibiting faster ribulose 1,5-bisphosphate regeneration and increased fluxes through the lower glycolysis and anaplerotic pathways towards the tricarboxylic acid cycle, amino acid synthesis and lipid synthesis than in higher plants.
Keywords
Article, /631/449, /631/1647/320, /631/449/1734, article
Sponsorship
Human Frontier Science Program (HFSP) (LT000156/2018, RGP0046/2018, RGP0046/2018)
Identifiers
s41477-021-01042-5, 1042
External DOI: https://doi.org/10.1038/s41477-021-01042-5
This record's URL: https://www.repository.cam.ac.uk/handle/1810/333298
Rights
Licence:
http://creativecommons.org/licenses/by/4.0/
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