Differential Requirement for the Cell Wall Integrity Sensor Wsc1p in Diploids Versus Haploids.
View / Open Files
Publication Date
2021-12-08Journal Title
J Fungi (Basel)
ISSN
2309-608X
Publisher
MDPI AG
Language
eng
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Hall, A. E., Lisci, M., & Rose, M. D. (2021). Differential Requirement for the Cell Wall Integrity Sensor Wsc1p in Diploids Versus Haploids.. J Fungi (Basel) https://doi.org/10.3390/jof7121049
Abstract
The primary role of the Cell Wall Integrity Pathway (CWI) in Saccharomyces cerevisiae is monitoring the state of the cell wall in response to general life cycle stresses (growth and mating) and imposed stresses (temperature changes and chemicals). Of the five mechanosensor proteins monitoring cell wall stress, Wsc1p and Mid2p are the most important. We find that WSC1 has a stringent requirement in zygotes and diploids, unlike haploids, and differing from MID2's role in shmoos. Diploids lacking WSC1 die frequently, independent of mating type. Death is due to loss of cell wall and plasma membrane integrity, which is suppressed by osmotic support. Overexpression of several CWI pathway components suppress wsc1∆ zygotic death, including WSC2, WSC3, and BEM2, as well as the Rho-GAPS, BEM3 and RGD2. Microscopic observations and suppression by BEM2 and BEM3 suggest that wsc1∆ zygotes die during bud emergence. Downstream in the CWI pathway, overexpression of a hyperactive protein kinase C (Pkc1p-R398P) causes growth arrest, and blocks the pheromone response. With moderate levels of Pkc1p-R398P, cells form zygotes and the wsc1∆ defect is suppressed. This work highlights functional differences in the requirement for Wsc1p in diploids Versus haploids and between Mid2p and Wsc1p during mating.
Keywords
Conjugation, Yeast, Ploidy, Protein kinase C, Lysis, Cell Wall Integrity
Sponsorship
NIH HHS (GM007388, R35GM126998)
National Institute of Health (R01GM037739)
Identifiers
34947031, PMC8703914
External DOI: https://doi.org/10.3390/jof7121049
This record's URL: https://www.repository.cam.ac.uk/handle/1810/333329
Statistics
Total file downloads (since January 2020). For more information on metrics see the
IRUS guide.
Recommended or similar items
The current recommendation prototype on the Apollo Repository will be turned off on 03 February 2023. Although the pilot has been fruitful for both parties, the service provider IKVA is focusing on horizon scanning products and so the recommender service can no longer be supported. We recognise the importance of recommender services in supporting research discovery and are evaluating offerings from other service providers. If you would like to offer feedback on this decision please contact us on: support@repository.cam.ac.uk