Transcriptional changes in the mammary gland during lactation revealed by single cell sequencing of cells from human milk
Authors
Engelbrecht, Lisa K
Bach, Karsten
Pensa, Sara
Stenning, Jack
Petricca, Stefania
Scheel, Christina H
Publication Date
2022-12Journal Title
Nature Communications
Publisher
Springer Science and Business Media LLC
Volume
13
Issue
1
Language
en
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Twigger, A., Engelbrecht, L. K., Bach, K., Schultz-Pernice, I., Pensa, S., Stenning, J., Petricca, S., et al. (2022). Transcriptional changes in the mammary gland during lactation revealed by single cell sequencing of cells from human milk. Nature Communications, 13 (1) https://doi.org/10.1038/s41467-021-27895-0
Abstract
<jats:title>Abstract</jats:title><jats:p>Under normal conditions, the most significant expansion and differentiation of the adult mammary gland occurs in response to systemic reproductive hormones during pregnancy and lactation to enable milk synthesis and secretion to sustain the offspring. However, human mammary tissue remodelling that takes place during pregnancy and lactation remains poorly understood due to the challenge of acquiring samples. We report here single-cell transcriptomic analysis of 110,744 viable breast cells isolated from human milk or non-lactating breast tissue, isolated from nine and seven donors, respectively. We found that human milk largely contains epithelial cells belonging to the luminal lineage and a repertoire of immune cells. Further transcriptomic analysis of the milk cells identified two distinct secretory cell types that shared similarities with luminal progenitors, but no populations comparable to hormone-responsive cells. Taken together, our data offers a reference map and a window into the cellular dynamics that occur during human lactation and may provide further insights on the interplay between pregnancy, lactation and breast cancer.</jats:p>
Keywords
Article, /631/1647/514/1949, /631/80/86, /631/443/494, /692/699/67/1347, /631/61/514/1949, /38/91, /13/31, /13/106, article
Sponsorship
RCUK | Medical Research Council (MRC) (MR/S036059/1)
Identifiers
s41467-021-27895-0, 27895
External DOI: https://doi.org/10.1038/s41467-021-27895-0
This record's URL: https://www.repository.cam.ac.uk/handle/1810/333395
Rights
Licence:
http://creativecommons.org/licenses/by/4.0/
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