Show simple item record

dc.contributor.authorPogodalla, Nicole
dc.contributor.authorKranenburg, Holger
dc.contributor.authorRey, Simone
dc.contributor.authorRodrigues, Silke
dc.contributor.authorCardona, Albert
dc.contributor.authorKlämbt, Christian
dc.date.accessioned2022-02-01T16:42:43Z
dc.date.available2022-02-01T16:42:43Z
dc.date.issued2021-11-04
dc.date.submitted2020-11-18
dc.identifier.issn2041-1723
dc.identifier.others41467-021-26462-x
dc.identifier.other26462
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/333502
dc.description.abstractIn the central nervous system (CNS), functional tasks are often allocated to distinct compartments. This is also evident in the Drosophila CNS where synapses and dendrites are clustered in distinct neuropil regions. The neuropil is separated from neuronal cell bodies by ensheathing glia, which as we show using dye injection experiments, contribute to the formation of an internal diffusion barrier. We find that ensheathing glia are polarized with a basolateral plasma membrane rich in phosphatidylinositol-(3,4,5)-triphosphate (PIP3) and the Na+/K+-ATPase Nervana2 (Nrv2) that abuts an extracellular matrix formed at neuropil-cortex interface. The apical plasma membrane is facing the neuropil and is rich in phosphatidylinositol-(4,5)-bisphosphate (PIP2) that is supported by a sub-membranous ßHeavy-Spectrin cytoskeleton. ßHeavy-spectrin mutant larvae affect ensheathing glial cell polarity with delocalized PIP2 and Nrv2 and exhibit an abnormal locomotion which is similarly shown by ensheathing glia ablated larvae. Thus, polarized glia compartmentalizes the brain and is essential for proper nervous system function.
dc.languageen
dc.publisherSpringer Science and Business Media LLC
dc.subjectArticle
dc.subject/631/378/87
dc.subject/631/378/1341
dc.subject/631/378/2596
dc.subject/64/24
dc.subject/38
dc.subject/38/1
dc.subject/38/89
dc.subject/38/35
dc.subjectarticle
dc.titleDrosophila ßHeavy-Spectrin is required in polarized ensheathing glia that form a diffusion-barrier around the neuropil.
dc.typeArticle
dc.date.updated2022-02-01T16:42:41Z
prism.issueIdentifier1
prism.publicationNameNat Commun
prism.volume12
dc.identifier.doi10.17863/CAM.80922
dcterms.dateAccepted2021-10-06
rioxxterms.versionofrecord10.1038/s41467-021-26462-x
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidCardona, Albert [0000-0003-4941-6536]
dc.contributor.orcidKlämbt, Christian [0000-0002-6349-5800]
dc.identifier.eissn2041-1723
cam.issuedOnline2021-11-04


Files in this item

Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record