Retaining the structural integrity of disulfide bonds in diphtheria toxoid carrier protein is crucial for effectiveness of glycoconjugate vaccine candidates
Del Bino, L
Dello Iacono, L
Royal Society of Chemistry
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Lopes Bernardes, G., carboni, F., kitowski, A., Sorieul, C., Veggi, D., Marques, M., Oldrini, D., et al. (2022). Retaining the structural integrity of disulfide bonds in diphtheria toxoid carrier protein is crucial for effectiveness of glycoconjugate vaccine candidates. Chemical Science https://doi.org/10.1039/D1SC01928G
The introduction of glycoconjugate vaccines marks an important point in the fight against various infectious diseases. The covalent conjugation of relevant polysaccharide antigens to immunogenic carrier proteins enables the induction of a long-lasting and robust IgG antibody response, which is not observed for pure polysaccharide vaccines. Although there has been remarkable progress in the development of glycoconjugate vaccines, many crucial parameters remain poorly understood. In particular, the influence of the conjugation site and strategy on immunogenic properties of the final glycoconjugate vaccine is the focus of intense research. Here, we present a comparison of two cysteine selective conjugation strategies, elucidating the impact of both modifications on the structural integrity of the carrier protein, as well as on the immunogenic properties of the resulting glycoconjugate vaccine candidates. Our work suggests that conjugation chemistries impairing structurally relevant elements of the protein carrier, such disulfide bonds, can have a dramatic effect on protein immunogenicity.
Marie Skłodowska-Curie grant agreement No. 675671. CS is recipient of the the grant No 861194 (PAVax) under EU Horizon 2020 program.
External DOI: https://doi.org/10.1039/D1SC01928G
This record's URL: https://www.repository.cam.ac.uk/handle/1810/333668
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by-nc/4.0/