Physical mechanisms of ESCRT-III-driven cell division.
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Authors
Hafner, Anne E
Vanhille-Campos, Christian
Pulschen, Andre
Hryniuk, Dawid
Culley, Siân
Baum, Buzz
Publication Date
2022-01-04Journal Title
Proc Natl Acad Sci U S A
ISSN
0027-8424
Publisher
Proceedings of the National Academy of Sciences
Volume
119
Issue
1
Language
eng
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Harker-Kirschneck, L., Hafner, A. E., Yao, T., Vanhille-Campos, C., Jiang, X., Pulschen, A., Hurtig, F., et al. (2022). Physical mechanisms of ESCRT-III-driven cell division.. Proc Natl Acad Sci U S A, 119 (1) https://doi.org/10.1073/pnas.2107763119
Abstract
Living systems propagate by undergoing rounds of cell growth and division. Cell division is at heart a physical process that requires mechanical forces, usually exerted by assemblies of cytoskeletal polymers. Here we developed a physical model for the ESCRT-III-mediated division of archaeal cells, which despite their structural simplicity share machinery and evolutionary origins with eukaryotes. By comparing the dynamics of simulations with data collected from live cell imaging experiments, we propose that this branch of life uses a previously unidentified division mechanism. Active changes in the curvature of elastic cytoskeletal filaments can lead to filament perversions and supercoiling, to drive ring constriction and deform the overlying membrane. Abscission is then completed following filament disassembly. The model was also used to explore how different adenosine triphosphate (ATP)-driven processes that govern the way the structure of the filament is changed likely impact the robustness and symmetry of the resulting division. Comparisons between midcell constriction dynamics in simulations and experiments reveal a good agreement with the process when changes in curvature are implemented at random positions along the filament, supporting this as a possible mechanism of ESCRT-III-dependent division in this system. Beyond archaea, this study pinpoints a general mechanism of cytokinesis based on dynamic coupling between a coiling filament and the membrane.
Keywords
Cell division, Archaea, Soft Matter, Escrt-iii, Membrane Simulations
Sponsorship
Wellcome Trust (203276/Z/16/Z)
European Research Council (802960)
Identifiers
34983838, PMC8740586
External DOI: https://doi.org/10.1073/pnas.2107763119
This record's URL: https://www.repository.cam.ac.uk/handle/1810/333691
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