The Pathological G51D Mutation in Alpha-Synuclein Oligomers Confers Distinct Structural Attributes and Cellular Toxicity.
Xu, Catherine K
Chen, Serene W
Dobson, Christopher M
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Xu, C. K., Castellana-Cruz, M., Chen, S. W., Du, Z., Meisl, G., Levin, A., Mannini, B., et al. (2022). The Pathological G51D Mutation in Alpha-Synuclein Oligomers Confers Distinct Structural Attributes and Cellular Toxicity.. Molecules https://doi.org/10.3390/molecules27041293
A wide variety of oligomeric structures are formed during the aggregation of proteins associated with neurodegenerative diseases. Such soluble oligomers are believed to be key toxic species in the related disorders; therefore, identification of the structural determinants of toxicity is of upmost importance. Here, we analysed toxic oligomers of α-synuclein and its pathological variants in order to identify structural features that could be related to toxicity and found a novel structural polymorphism within G51D oligomers. These G51D oligomers can adopt a variety of β-sheet-rich structures with differing degrees of α-helical content, and the helical structural content of these oligomers correlates with the level of induced cellular dysfunction in SH-SY5Y cells. This structure-function relationship observed in α-synuclein oligomers thus presents the α-helical structure as another potential structural determinant that may be linked with cellular toxicity in amyloid-related proteins.
Wellcome Trust (094425/Z/10/Z)
External DOI: https://doi.org/10.3390/molecules27041293
This record's URL: https://www.repository.cam.ac.uk/handle/1810/333877
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/