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dc.contributor.authorMak, Christopher CH
dc.contributor.authorTo, Kendrick
dc.contributor.authorFekir, Karim
dc.contributor.authorBrooks, Roger
dc.contributor.authorKhan, Wasim
dc.date.accessioned2022-02-12T16:20:47Z
dc.date.available2022-02-12T16:20:47Z
dc.date.issued2022-02-12
dc.date.submitted2021-08-09
dc.identifier.issn1478-811X
dc.identifier.others12964-021-00815-x
dc.identifier.other815
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/333963
dc.description.abstractAIM: The variable results in clinical trials of adipose tissue-derived stem cells (ASCs) for chondral defects may be due to the different ex vivo culture conditions of the ASCs which are implanted to treat the lesions. We sought to determine the optimal in vitro chondrocyte co-culture condition that promotes infrapatellar fat pad-derived (IFPD) ASC chondrogenic gene expression in a novel co-culture combination. METHODS: In our study, we utilized an in vitro autologous co-culture of IFPD ASCs and articular chondrocytes derived from Kellgren-Lawrence Grade III/IV osteoarthritic human knee joints at ASC-to-chondrocyte seeding log ratios of 1:1, 10:1, and 100:1. Gene expression following in vitro co-culture was quantified by RT-qPCR with a panel comprising COL1A1, COL2A1, COL10A1, L-SOX5, SOX6, SOX9, ACAN, HSPG2, and COMP for chondrogenic gene expression. RESULTS: The chondrogenic gene expression profiles from co-cultures were greater than would be expected from an expression profile modeled from chondrocyte and ASC-only monocultures. Additionally, chondrogenic gene expression decreased with increasing ASC-to-chondrocyte seeding ratios. CONCLUSIONS: These findings provide insight into the mechanisms underlying clinical ASC therapies and signifies that IFPD ASCs pre-conditioned by chondrocyte co-culture may have improved chondrogenic potential for cartilage repair. This model can help further understand IFPD ASCs in chondral and osteochondral repair and the chondrogenic pathways involved. Video Abstract.
dc.languageen
dc.publisherSpringer Science and Business Media LLC
dc.subjectShort Report
dc.subjectMesenchymal stromal cells
dc.subjectCo-culture
dc.subjectChondrogenesis
dc.subjectCartilage
dc.subjectCell-based therapy
dc.subjectRegenerative medicine
dc.subjectOsteoarthritis
dc.titleInfrapatellar fat pad adipose-derived stem cells co-cultured with articular chondrocytes from osteoarthritis patients exhibit increased chondrogenic gene expression.
dc.typeArticle
dc.date.updated2022-02-12T16:20:45Z
prism.issueIdentifier1
prism.publicationNameCell Commun Signal
prism.volume20
dc.identifier.doi10.17863/CAM.81380
dcterms.dateAccepted2021-12-06
rioxxterms.versionofrecord10.1186/s12964-021-00815-x
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
dc.contributor.orcidTo, Kendrick [0000-0001-9262-7190]
dc.contributor.orcidKhan, Wasim [0000-0003-3106-5424]
dc.identifier.eissn1478-811X
pubs.funder-project-idARTHRITIS RESEARCH UK (21156)
cam.issuedOnline2022-02-12


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