B cell receptor repertoire kinetics after SARS-CoV-2 infection and vaccination.
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Authors
Kotagiri, Prasanti
Mescia, Federica
Rae, William M
Bergamaschi, Laura
Turner, Lorinda
Hunter, Kelvin
Gerber, Pehuén P
Cambridge Institute of Therapeutic Immunology and Infectious Disease-National Institute of Health Research (CITIID-NIHR) COVID BioResource Collaboration
Hess, Christoph
Goodfellow, Ian G
McKinney, Eoin F
Bashford-Rogers, Rachael JM
Publication Date
2022-01-31Journal Title
Cell Reports
ISSN
2211-1247
Publisher
Elsevier
Number
110393
Pages
110393
Type
Article
This Version
AM
Physical Medium
Print-Electronic
Metadata
Show full item recordCitation
Kotagiri, P., Mescia, F., Rae, W. M., Bergamaschi, L., Tuong, K., Turner, L., Hunter, K., et al. (2022). B cell receptor repertoire kinetics after SARS-CoV-2 infection and vaccination.. Cell Reports, (110393), 110393. https://doi.org/10.1016/j.celrep.2022.110393
Abstract
B cells are important in immunity to both severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection and vaccination, but B cell receptor (BCR) repertoire development in these contexts has not been compared. We analyze serial samples from 171 SARS-CoV-2-infected individuals and 63 vaccine recipients and find the global BCR repertoire differs between them. Following infection, immunoglobulin (Ig)G1/3 and IgA1 BCRs increase, somatic hypermutation (SHM) decreases, and, in severe disease, IgM and IgA clones are expanded. In contrast, after vaccination, the proportion of IgD/M BCRs increase, SHM is unchanged, and expansion of IgG clones is prominent. VH1-24, which targets the N-terminal domain (NTD) and contributes to neutralization, is expanded post infection except in the most severe disease. Infection generates a broad distribution of SARS-CoV-2-specific clones predicted to target the spike protein, while a more focused response after vaccination mainly targets the spike's receptor-binding domain. Thus, the nature of SARS-CoV-2 exposure differentially affects BCR repertoire development, potentially informing vaccine strategies.
Keywords
B cell receptor repertoire, COVID-19, SARS-CoV-2 vaccination
Sponsorship
Wellcome Trust (200871/Z/16/Z)
Medical Research Council (MR/P008801/1)
Wellcome Trust (207498/Z/17/Z)
Medical Research Council (MR/S035842/1)
Identifiers
External DOI: https://doi.org/10.1016/j.celrep.2022.110393
This record's URL: https://www.repository.cam.ac.uk/handle/1810/334027
Rights
Publisher's own licence
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