Arabidopsis thaliana CYCLIC NUCLEOTIDE-GATED CHANNEL2 mediates extracellular ATP signal transduction in root epidermis.
Authors
Ning, Youzheng
McNelly, Rose E
Dark, Adeeba
Publication Date
2022-04Journal Title
New Phytol
ISSN
0028-646X
Publisher
Wiley
Language
en
Type
Other
This Version
AO
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Wang, L., Ning, Y., Sun, J., Wilkins, K. A., Matthus, E., McNelly, R. E., Dark, A., et al. (2022). Arabidopsis thaliana CYCLIC NUCLEOTIDE-GATED CHANNEL2 mediates extracellular ATP signal transduction in root epidermis.. [Other]. https://doi.org/10.1111/nph.17987
Description
Funder: Agence Nationale de la Recherche; Id: http://dx.doi.org/10.13039/501100001665
Funder: Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada; Id: http://dx.doi.org/10.13039/501100002790
Abstract
Damage can be signalled by extracellular ATP (eATP) using plasma membrane (PM) receptors to effect cytosolic free calcium ion ([Ca2+ ]cyt ) increase as a second messenger. The downstream PM Ca2+ channels remain enigmatic. Here, the Arabidopsis thaliana Ca2+ channel subunit CYCLIC NUCLEOTIDE-GATED CHANNEL2 (CNGC2) was identified as a critical component linking eATP receptors to downstream [Ca2+ ]cyt signalling in roots. Extracellular ATP-induced changes in single epidermal cell PM voltage and conductance were measured electrophysiologically, changes in root [Ca2+ ]cyt were measured with aequorin, and root transcriptional changes were determined by quantitative real-time PCR. Two cngc2 loss-of-function mutants were used: cngc2-3 and defence not death1 (which expresses cytosolic aequorin). Extracellular ATP-induced transient depolarization of Arabidopsis root elongation zone epidermal PM voltage was Ca2+ dependent, requiring CNGC2 but not CNGC4 (its channel co-subunit in immunity signalling). Activation of PM Ca2+ influx currents also required CNGC2. The eATP-induced [Ca2+ ]cyt increase and transcriptional response in cngc2 roots were significantly impaired. CYCLIC NUCLEOTIDE-GATED CHANNEL2 is required for eATP-induced epidermal Ca2+ influx, causing depolarization leading to [Ca2+ ]cyt increase and damage-related transcriptional response.
Keywords
Rapid report, Rapid reports, Arabidopsis, calcium, CNGC4, CYCLIC NUCLEOTIDE‐GATED CHANNEL2 (CNGC2), depolarization, extracellular ATP, plasma membrane potential, root epidermis
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/S004637/1)
Biotechnology and Biological Sciences Research Council (BB/J014540/1)
Identifiers
nph17987, 2021-37680
External DOI: https://doi.org/10.1111/nph.17987
This record's DOI: https://doi.org/10.17863/CAM.81663
Rights
Licence:
http://creativecommons.org/licenses/by/4.0/
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