Monitoring the interactions between alpha-synuclein and Tau in vitro and in vivo using bimolecular fluorescence complementation.
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Authors
Torres-Garcia, Laura
P Domingues, Joana M
Brandi, Edoardo
Haikal, Caroline
Mudannayake, Janitha M
Brás, Inês C
Gerhardt, Ellen
Li, Wen
Svanbergsson, Alexander
Outeiro, Tiago F
Li, Jia-Yi
Publication Date
2022-02-22Journal Title
Sci Rep
ISSN
2045-2322
Publisher
Springer Science and Business Media LLC
Volume
12
Issue
1
Language
eng
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Torres-Garcia, L., P Domingues, J. M., Brandi, E., Haikal, C., Mudannayake, J. M., Brás, I. C., Gerhardt, E., et al. (2022). Monitoring the interactions between alpha-synuclein and Tau in vitro and in vivo using bimolecular fluorescence complementation.. Sci Rep, 12 (1) https://doi.org/10.1038/s41598-022-06846-9
Abstract
Parkinson's disease (PD) and Alzheimer's disease (AD) are characterized by pathological accumulation and aggregation of different amyloidogenic proteins, α-synuclein (aSyn) in PD, and amyloid-β (Aβ) and Tau in AD. Strikingly, few PD and AD patients' brains exhibit pure pathology with most cases presenting mixed types of protein deposits in the brain. Bimolecular fluorescence complementation (BiFC) is a technique based on the complementation of two halves of a fluorescent protein, which allows direct visualization of protein-protein interactions. In the present study, we assessed the ability of aSyn and Tau to interact with each other. For in vitro evaluation, HEK293 and human neuroblastoma cells were used, while in vivo studies were performed by AAV6 injection in the substantia nigra pars compacta (SNpc) of mice and rats. We observed that the co-expression of aSyn and Tau led to the emergence of fluorescence, reflecting the interaction of the proteins in cell lines, as well as in mouse and rat SNpc. Thus, our data indicates that aSyn and Tau are able to interact with each other in a biologically relevant context, and that the BiFC assay is an effective tool for studying aSyn-Tau interactions in vitro and in different rodent models in vivo.
Keywords
Cell Line, Tumor, Animals, Humans, Mice, Rats, tau Proteins, Fluorescent Antibody Technique, Fluorescence, alpha-Synuclein, HEK293 Cells, Protein Interaction Maps, In Vitro Techniques, Protein Aggregates
Identifiers
35194057, PMC8863885
External DOI: https://doi.org/10.1038/s41598-022-06846-9
This record's URL: https://www.repository.cam.ac.uk/handle/1810/335414
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