Stress-associated developmental reprogramming in moss protonemata by synthetic activation of the common symbiosis pathway.
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Authors
Kleist, Thomas J
Bortolazzo, Anthony
Keyser, Zachary P
Perera, Adele M
Irving, Thomas B
Venkateshwaran, Muthusubramanian
Atanjaoui, Fatiha
Tang, Ren-Jie
Maeda, Junko
Cartwright, Heather N
Christianson, Michael L
Lemaux, Peggy G
Luan, Sheng
Ané, Jean-Michel
Publication Date
2022-02-18Journal Title
iScience
ISSN
2589-0042
Publisher
Elsevier BV
Volume
25
Issue
2
Number
103754
Pages
103754
Type
Article
This Version
VoR
Physical Medium
Electronic-eCollection
Metadata
Show full item recordCitation
Kleist, T. J., Bortolazzo, A., Keyser, Z. P., Perera, A. M., Irving, T. B., Venkateshwaran, M., Atanjaoui, F., et al. (2022). Stress-associated developmental reprogramming in moss protonemata by synthetic activation of the common symbiosis pathway.. iScience, 25 (2. 103754), 103754. https://doi.org/10.1016/j.isci.2022.103754
Abstract
Symbioses between angiosperms and rhizobia or arbuscular mycorrhizal fungi are controlled through a conserved signaling pathway. Microbe-derived, chitin-based elicitors activate plant cell surface receptors and trigger nuclear calcium oscillations, which are decoded by a calcium/calmodulin-dependent protein kinase (CCaMK) and its target transcription factor interacting protein of DMI3 (IPD3). Genes encoding CCaMK and IPD3 have been lost in multiple non-mycorrhizal plant lineages yet retained among non-mycorrhizal mosses. Here, we demonstrated that the moss Physcomitrium is equipped with a bona fide CCaMK that can functionally complement a Medicago loss-of-function mutant. Conservation of regulatory phosphosites allowed us to generate predicted hyperactive forms of Physcomitrium CCaMK and IPD3. Overexpression of synthetically activated CCaMK or IPD3 in Physcomitrium led to abscisic acid (ABA) accumulation and ectopic development of brood cells, which are asexual propagules that facilitate escape from local abiotic stresses. We therefore propose a functional role for Physcomitrium CCaMK-IPD3 in stress-associated developmental reprogramming.
Keywords
Developmental biology, Mycology, Plant biology
Identifiers
External DOI: https://doi.org/10.1016/j.isci.2022.103754
This record's URL: https://www.repository.cam.ac.uk/handle/1810/335459
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