Co-translational assembly orchestrates competing biogenesis pathways.
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Authors
Becker, Anja
Pereira, Filipa
Landry, Jonathan JM
de Azevedo, Nayara Trevisan Doimo
Kaindl, Eva
Romanov, Natalie
Baumbach, Janina
Patil, Kiran Raosaheb
Publication Date
2022-03-09Journal Title
Nat Commun
ISSN
2041-1723
Publisher
Springer Science and Business Media LLC
Volume
13
Issue
1
Language
eng
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Seidel, M., Becker, A., Pereira, F., Landry, J. J., de Azevedo, N. T. D., Fusco, C. M., Kaindl, E., et al. (2022). Co-translational assembly orchestrates competing biogenesis pathways.. Nat Commun, 13 (1) https://doi.org/10.1038/s41467-022-28878-5
Abstract
During the co-translational assembly of protein complexes, a fully synthesized subunit engages with the nascent chain of a newly synthesized interaction partner. Such events are thought to contribute to productive assembly, but their exact physiological relevance remains underexplored. Here, we examine structural motifs contained in nucleoporins for their potential to facilitate co-translational assembly. We experimentally test candidate structural motifs and identify several previously unknown co-translational interactions. We demonstrate by selective ribosome profiling that domain invasion motifs of beta-propellers, coiled-coils, and short linear motifs may act as co-translational assembly domains. Such motifs are often contained in proteins that are members of multiple complexes (moonlighters) and engage with closely related paralogs. Surprisingly, moonlighters and paralogs assemble co-translationally in only some but not all of the relevant biogenesis pathways. Our results highlight the regulatory complexity of assembly pathways.
Sponsorship
European Research Council (724349, 743216)
Identifiers
35264577, PMC8907234
External DOI: https://doi.org/10.1038/s41467-022-28878-5
This record's URL: https://www.repository.cam.ac.uk/handle/1810/335963
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