Microtubule rescue at midzone edges promotes overlap stability and prevents spindle collapse during anaphase B.
Publication Date
2022-03-16Journal Title
Elife
ISSN
2050-084X
Publisher
eLife Sciences Publications, Ltd
Volume
11
Language
en
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Lera-Ramirez, M., Nédélec, F. J., & Tran, P. T. (2022). Microtubule rescue at midzone edges promotes overlap stability and prevents spindle collapse during anaphase B.. Elife, 11 https://doi.org/10.7554/eLife.72630
Description
Funder: Gatsby Charitable Foundation; FundRef: http://dx.doi.org/10.13039/501100000324
Funder: Institut National Du Cancer; FundRef: http://dx.doi.org/10.13039/501100006364
Funder: Fondation ARC pour la Recherche sur le Cancer; FundRef: http://dx.doi.org/10.13039/501100004097
Funder: La Ligue Contre le Cancer - Ile de France
Abstract
During anaphase B, molecular motors slide interpolar microtubules to elongate the mitotic spindle, contributing to the separation of chromosomes. However, sliding of antiparallel microtubules reduces their overlap, which may lead to spindle breakage, unless microtubules grow to compensate sliding. How sliding and growth are coordinated is still poorly understood. In this study, we have used the fission yeast S. pombe to measure microtubule dynamics during anaphase B. We report that the coordination of microtubule growth and sliding relies on promoting rescues at the midzone edges. This makes microtubules stable from pole to midzone, while their distal parts including the plus ends alternate between assembly and disassembly. Consequently, the midzone keeps a constant length throughout anaphase, enabling sustained sliding without the need for a precise regulation of microtubule growth speed. Additionally, we found that in S. pombe, which undergoes closed mitosis, microtubule growth speed decreases when the nuclear membrane wraps around the spindle midzone.
Keywords
Research Article, Cell Biology, microtubule dynamics, anaphase, spindle, microtubule sliding, modelling, live cell imaging, S. pombe
Sponsorship
H2020 Marie Skłodowska-Curie Actions (675737)
Identifiers
72630
External DOI: https://doi.org/10.7554/eLife.72630
This record's URL: https://www.repository.cam.ac.uk/handle/1810/336252
Rights
Licence:
http://creativecommons.org/licenses/by/4.0/
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