Current methods to analyze lysosome morphology, positioning, motility and function.
Authors
Cutler, Dan F
Futter, Clare E
Galione, Antony
Medina, Diego Luis
Napolitano, Gennaro
Settembre, Carmine
Aerts, Johannes MFG
Atakpa-Adaji, Peace
Bruno, Gemma
Capuozzo, Antonella
De Leonibus, Elvira
Di Malta, Chiara
Esposito, Alessandra
Grumati, Paolo
Monfregola, Jlenia
Montefusco, Sandro
De Risi, Maria
Soldati, Chiara
Publication Date
2022-05Journal Title
Traffic
ISSN
1398-9219
Publisher
Wiley
Language
en
Type
Article
This Version
AO
VoR
Metadata
Show full item recordCitation
Barral, D. C., Staiano, L., Guimas Almeida, C., Cutler, D. F., Eden, E. R., Futter, C. E., Galione, A., et al. (2022). Current methods to analyze lysosome morphology, positioning, motility and function.. Traffic https://doi.org/10.1111/tra.12839
Description
Funder: Maratona da Saúde
Funder: Royal Society Wolfson
Funder: Wellcome; Id: http://dx.doi.org/10.13039/100010269
Abstract
Since the discovery of lysosomes more than 70 years ago, much has been learned about the functions of these organelles. Lysosomes were regarded as exclusively degradative organelles, but more recent research has shown that they play essential roles in several other cellular functions, such as nutrient sensing, intracellular signalling and metabolism. Methodological advances played a key part in generating our current knowledge about the biology of this multifaceted organelle. In this review, we cover current methods used to analyze lysosome morphology, positioning, motility and function. We highlight the principles behind these methods, the methodological strategies and their advantages and limitations. To extract accurate information and avoid misinterpretations, we discuss the best strategies to identify lysosomes and assess their characteristics and functions. With this review, we aim to stimulate an increase in the quantity and quality of research on lysosomes and further ground-breaking discoveries on an organelle that continues to surprise and excite cell biologists.
Keywords
REVIEW, REVIEWS, endolysosomes, lysosomal storage diseases, lysosome biogenesis, lysosome exocytosis, lysosome‐related organelles, lysosomes, membrane contact sites, mTOR, TFEB
Sponsorship
Fundação para a Ciência e a Tecnologia (CEECIND/00410/2017)
H2020 Spreading Excellence and Widening Participation (811087)
Identifiers
tra12839
External DOI: https://doi.org/10.1111/tra.12839
This record's URL: https://www.repository.cam.ac.uk/handle/1810/336413
Rights
Licence:
http://creativecommons.org/licenses/by/4.0/
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