Intestinal enteroendocrine cell signaling: Retinol-binding protein 2 (RBP2) and retinoid actions
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Authors
Calderon, Rossana M
Smith, Christopher A
Miedzybrodzka, Emily L
Silvaroli, Josie A
Golczak, Marcin
Gribble, Fiona M
Blaner, William S
Journal Title
Endocrinology
ISSN
0013-7227
Publisher
Endocrine Society
Type
Article
This Version
AM
Metadata
Show full item recordCitation
Reimann, F., Calderon, R. M., Smith, C. A., Miedzybrodzka, E. L., Silvaroli, J. A., Golczak, M., Gribble, F. M., & et al. Intestinal enteroendocrine cell signaling: Retinol-binding protein 2 (RBP2) and retinoid actions. Endocrinology https://doi.org/10.17863/CAM.84379
Abstract
Retinol-binding protein 2-deficient (Rbp2-/-) mice are more prone to obesity, glucose intolerance, and hepatic steatosis than matched controls. Glucose-dependent insulinotropic polypeptide (GIP) blood levels are dysregulated in these mice. The present studies provide new insights into these observations. Single cell transcriptomic and immunohistochemical studies establish that RBP2 is highly expressed in enteroendocrine cells (EECs) that produce incretins, either GIP or glucagon-like peptide-1 (GLP-1). EECs also express an enzyme needed for all-trans-retinoic acid (ATRA) synthesis, ALDH1A1, and retinoic acid receptor-α (RARα), which mediates ATRA-dependent transcription. Total and GIP-positive EECs are significantly lower in Rbp2-/- mice. The plasma transport protein for retinol, retinol-binding protein 4 (RBP4) is also expressed in EECs and is co-secreted with GIP upon stimulation. Collectively, our data support direct roles for RBP2 and ATRA in cellular processes that give rise to GIP-producing EECs and roles for RBP2 and RBP4 within EECs that facilitate hormone storage and secretion.
Sponsorship
Wellcome Trust (106262/Z/14/Z)
MRC (MC_UU_00014/3)
Embargo Lift Date
2025-05-09
Identifiers
This record's DOI: https://doi.org/10.17863/CAM.84379
This record's URL: https://www.repository.cam.ac.uk/handle/1810/336956
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