Quantitative analysis of protein-RNA interactions in fission yeast
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Publication Date
2022-06Journal Title
STAR Protocols
ISSN
2666-1667
Publisher
Elsevier BV
Volume
3
Issue
2
Number
101373
Pages
101373-101373
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Elías-Villalobos, A., Duncan, C., Mata, J., & Helmlinger, D. (2022). Quantitative analysis of protein-RNA interactions in fission yeast. STAR Protocols, 3 (2. 101373), 101373-101373. https://doi.org/10.1016/j.xpro.2022.101373
Abstract
Characterizing the interactions between RNAs and proteins in vivo is key to better understand how organisms regulate gene expression. Here, we describe a robust and quantitative protocol to measure specific RNA-protein interactions in a native context using RNA immunoprecipitation (RIP). We provide a comprehensive experimental framework to detect cotranslational interactions and detail the quantitative analysis of purified RNAs by PCR and high-throughput sequencing. Although we developed the protocol in fission yeast, it can be readily implemented in other yeast species.
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/G011869/1)
Biotechnology and Biological Sciences Research Council (BB/S015833/1)
Identifiers
External DOI: https://doi.org/10.1016/j.xpro.2022.101373
This record's URL: https://www.repository.cam.ac.uk/handle/1810/337102
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International
Licence URL: https://creativecommons.org/licenses/by-nc-nd/4.0/
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