Quantitative analysis of protein-RNA interactions in fission yeast
Published version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Elías-Villalobos, Alberto
Duncan, Caia
Mata, Juan https://orcid.org/0000-0002-5514-3653
Helmlinger, Dominique
Abstract
Characterizing the interactions between RNAs and proteins in vivo is key to better understand how organisms regulate gene expression. Here, we describe a robust and quantitative protocol to measure specific RNA-protein interactions in a native context using RNA immunoprecipitation (RIP). We provide a comprehensive experimental framework to detect cotranslational interactions and detail the quantitative analysis of purified RNAs by PCR and high-throughput sequencing. Although we developed the protocol in fission yeast, it can be readily implemented in other yeast species.
Description
Keywords
Model Organisms, Molecular Biology, Protein Biochemistry, Immunoprecipitation, Proteins, RNA, Schizosaccharomyces, Yeast, Dried
Journal Title
STAR Protocols
Conference Name
Journal ISSN
2666-1667
2666-1667
2666-1667
Volume Title
3
Publisher
Elsevier BV
Publisher DOI
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/G011869/1)
Biotechnology and Biological Sciences Research Council (BB/S015833/1)
Biotechnology and Biological Sciences Research Council (BB/S015833/1)