Comparative Genomics of Disease and Carriage Serotype 1 Pneumococci.
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Authors
Ebruke, Chinelo
Senghore, Madikay
Tientcheu, Peggy-Estelle
Gladstone, Rebecca A
Tonkin-Hill, Gerry
Cornick, Jennifer E
Yang, Marie
Worwui, Archibald
McGee, Lesley
Breiman, Robert F
Klugman, Keith P
Kadioglu, Aras
Mackenzie, Grant
Croucher, Nicholas J
Roca, Anna
Kwambana-Adams, Brenda A
Antonio, Martin
Bentley, Stephen D
Publication Date
2022-04-10Journal Title
Genome Biol Evol
ISSN
1759-6653
Publisher
Oxford University Press (OUP)
Language
eng
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Chaguza, C., Ebruke, C., Senghore, M., Lo, S. W., Tientcheu, P., Gladstone, R. A., Tonkin-Hill, G., et al. (2022). Comparative Genomics of Disease and Carriage Serotype 1 Pneumococci.. Genome Biol Evol https://doi.org/10.1093/gbe/evac052
Abstract
The isolation of Streptococcus pneumoniae serotypes in systemic tissues of patients with invasive disease versus the nasopharynx of healthy individuals with asymptomatic carriage varies widely. Some serotypes are hyper-invasive, particularly serotype 1, but the underlying genetics remain poorly understood due to the rarity of carriage isolates, reducing the power of comparison with invasive isolates. Here, we use a well-controlled genome-wide association study to search for genetic variation associated with invasiveness of serotype 1 pneumococci from a serotype 1 endemic setting in Africa. We found no consensus evidence that certain genomic variation is overrepresented among isolates from patients with invasive disease than asymptomatic carriage. Overall, the genomic variation explained negligible phenotypic variability, suggesting a minimal effect on the disease status. Furthermore, changes in lineage distribution were seen with lineages replacing each other over time, highlighting the importance of continued pathogen surveillance. Our findings suggest that the hyper-invasiveness is an intrinsic property of the serotype 1 strains, not specific for a "disease-associated" subpopulation disproportionately harboring unique genomic variation.
Keywords
Streptococcus pneumoniae, bacterial genomics, genome-wide association study, genomic epidemiology, invasiveness, pathogenicity, Carrier State, Genome-Wide Association Study, Genomics, Humans, Nasopharynx, Pneumococcal Infections, Pneumococcal Vaccines, Serogroup, Streptococcus pneumoniae
Sponsorship
Wellcome Trust (206194)
Identifiers
35439297, PMC9048925
External DOI: https://doi.org/10.1093/gbe/evac052
This record's URL: https://www.repository.cam.ac.uk/handle/1810/337371
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