Synthetic biology and bioelectrochemical tools for electrogenetic system engineering.
Wey, Laura T
PixCell iGEM Team
American Association for the Advancement of Science (AAAS)
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Lawrence, J. M., Yin, Y., Bombelli, P., Scarampi, A., Storch, M., Wey, L. T., Climent-Catala, A., et al. (2022). Synthetic biology and bioelectrochemical tools for electrogenetic system engineering.. Sci Adv, 8 (18) https://doi.org/10.1126/sciadv.abm5091
Synthetic biology research and its industrial applications rely on deterministic spatiotemporal control of gene expression. Recently, electrochemical control of gene expression has been demonstrated in electrogenetic systems (redox-responsive promoters used alongside redox inducers and electrodes), allowing for the direct integration of electronics with biological processes. However, the use of electrogenetic systems is limited by poor activity, tunability, and standardization. In this work, we developed a strong, unidirectional, redox-responsive promoter before deriving a mutant promoter library with a spectrum of strengths. We constructed genetic circuits with these parts and demonstrated their activation by multiple classes of redox molecules. Last, we demonstrated electrochemical activation of gene expression under aerobic conditions using a novel, modular bioelectrochemical device. These genetic and electrochemical tools facilitate the design and improve the performance of electrogenetic systems. Furthermore, the genetic design strategies used can be applied to other redox-responsive promoters to further expand the available tools for electrogenetics.
Is supplemented by: https://doi.org/10.17863/CAM.78759
Biotechnology and Biological Sciences Research Council (BB/M011194/1)
Biotechnology and Biological Sciences Research Council (BB/R011923/1)
External DOI: https://doi.org/10.1126/sciadv.abm5091
This record's URL: https://www.repository.cam.ac.uk/handle/1810/337799
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/