Synthetic biology and bioelectrochemical tools for electrogenetic system engineering.
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Authors
Bombelli, Paolo
Wey, Laura T
PixCell iGEM Team
Publication Date
2022-05-06Journal Title
Sci Adv
ISSN
2375-2548
Publisher
American Association for the Advancement of Science (AAAS)
Volume
8
Issue
18
Language
eng
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Lawrence, J., Yin, Y., Bombelli, P., Scarampi, A., Storch, M., Wey, L. T., Climent-Catala, A., et al. (2022). Synthetic biology and bioelectrochemical tools for electrogenetic system engineering.. Sci Adv, 8 (18) https://doi.org/10.1126/sciadv.abm5091
Abstract
Synthetic biology research and its industrial applications rely on deterministic spatiotemporal control of gene expression. Recently, electrochemical control of gene expression has been demonstrated in electrogenetic systems (redox-responsive promoters used alongside redox inducers and electrodes), allowing for the direct integration of electronics with biological processes. However, the use of electrogenetic systems is limited by poor activity, tunability, and standardization. In this work, we developed a strong, unidirectional, redox-responsive promoter before deriving a mutant promoter library with a spectrum of strengths. We constructed genetic circuits with these parts and demonstrated their activation by multiple classes of redox molecules. Last, we demonstrated electrochemical activation of gene expression under aerobic conditions using a novel, modular bioelectrochemical device. These genetic and electrochemical tools facilitate the design and improve the performance of electrogenetic systems. Furthermore, the genetic design strategies used can be applied to other redox-responsive promoters to further expand the available tools for electrogenetics.
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/M011194/1)
Biotechnology and Biological Sciences Research Council (BB/R011923/1)
Identifiers
35507663, PMC9067924
External DOI: https://doi.org/10.1126/sciadv.abm5091
This record's URL: https://www.repository.cam.ac.uk/handle/1810/337799
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