Investigating the structural changes due to adenosine methylation of the Kaposi's sarcoma-associated herpes virus ORF50 transcript.
PLoS Comput Biol
Public Library of Science (PLoS)
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Röder, K., Barker, A. M., Whitehouse, A., & Pasquali, S. (2022). Investigating the structural changes due to adenosine methylation of the Kaposi's sarcoma-associated herpes virus ORF50 transcript.. PLoS Comput Biol, 18 (5) https://doi.org/10.1371/journal.pcbi.1010150
Funder: Cambridge Philosophical Society
Funder: Universite de Paris
Kaposi's sarcoma-associated herpes virus (KSHV) is a human oncovirus. KSHV relies on manipulating the host cell N6-methyl adenosine (m6A) RNA modification pathway to enhance virus replication. Methylation within a RNA stem loop of the open reading frame 50 (ORF50) increases transcript stability via the recruitment of the m6A reader, SND1. In this contribution we explore the energy landscapes of the unmethylated and methylated RNA stem loops of ORF50 to investigate the effect of methylation on the structure of the stem loop. We observe a significant shift upon methylation between an open and closed configuration of the top of the stem loop. In the unmethylated stem loop the closed configuration is much lower in energy, and, as a result, exhibits higher occupancy.
Cell Line, Humans, Herpesvirus 8, Human, Sarcoma, Kaposi, Endonucleases, RNA, Adenosine, Gene Expression Regulation, Viral, Methylation, Open Reading Frames
Biotechnology and Biological Sciences Research Council (BB/V006363/1, BB/T00021X/1)
Medical Research Foundation (MR/R010145/1)
Medical Research Council (MR/R010145/1)
External DOI: https://doi.org/10.1371/journal.pcbi.1010150
This record's URL: https://www.repository.cam.ac.uk/handle/1810/338555
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/