Lifespan and ROS levels in different Drosophila melanogaster strains after 24 h hypoxia exposure.
Authors
De Lazzari, Federica
Mrakic-Sposta, Simona
Vezzoli, Alessandra
Zordan, Mauro A
Bisaglia, Marco
Menti, Giulio Maria
Frighetto, Giovanni
Bosco, Gerardo
Dal Cappello, Tomas
Strapazzon, Giacomo
Reggiani, Carlo
Gussoni, Maristella
Megighian, Aram
Publication Date
2022-06-15Journal Title
Biol Open
ISSN
2046-6390
Publisher
The Company of Biologists
Volume
11
Issue
6
Language
en
Type
Article
This Version
AO
VoR
Metadata
Show full item recordCitation
Malacrida, S., De Lazzari, F., Mrakic-Sposta, S., Vezzoli, A., Zordan, M. A., Bisaglia, M., Menti, G. M., et al. (2022). Lifespan and ROS levels in different Drosophila melanogaster strains after 24 h hypoxia exposure.. Biol Open, 11 (6) https://doi.org/10.1242/bio.059386
Description
Funder: EURAC Research: Accademia Europea; Grant(s): 58006
Abstract
During recent decades, model organisms such as Drosophila melanogaster have made it possible to study the effects of different environmental oxygen conditions on lifespan and oxidative stress. However, many studies have often yielded controversial results usually assigned to variations in Drosophila genetic background and differences in study design. In this study, we compared longevity and ROS levels in young, unmated males of three laboratory wild-type lines (Canton-S, Oregon-R and Berlin-K) and one mutant line (Sod1n1) as a positive control of redox imbalance, under both normoxic and hypoxic (2% oxygen for 24 h) conditions. Lifespan was used to detect the effects of hypoxic treatment and differences were analysed by means of Kaplan-Meier survival curves and log-rank tests. Electron paramagnetic resonance spectroscopy was used to measure ROS levels and analysis of variance was used to estimate the effects of hypoxic treatment and to assess ROS differences between strains. We observed that the genetic background is a relevant factor involved in D. melanogaster longevity and ROS levels. Indeed, as expected, in normoxia Sod1n1 are the shortest-lived, while the wild-type strains, despite a longer lifespan, show some differences, with the Canton-S line displaying the lowest mortality rate. After hypoxic stress these variances are amplified, with Berlin-K flies showing the highest mortality rate and most evident reduction of lifespan. Moreover, our analysis highlighted differential effects of hypoxia on redox balance/unbalance. Canton-S flies had the lowest increase of ROS level compared to all the other strains, confirming it to be the less sensitive to hypoxic stress. Sod1n1 flies displayed the highest ROS levels in normoxia and after hypoxia. These results should be used to further standardize future Drosophila research models designed to investigate genes and pathways that may be involved in lifespan and/or ROS, as well as comparative studies on specific mutant strains.
Keywords
RESEARCH ARTICLE, Drosophila melanogaster, Wild-type strain, Hypoxia, Lifespan, ROS, EPR
Sponsorship
Department of Innovation, Research, University and Museums of the Autonomous Province of Bozen/Bolzano (8894/2022)
Identifiers
bio059386
External DOI: https://doi.org/10.1242/bio.059386
This record's URL: https://www.repository.cam.ac.uk/handle/1810/338609
Rights
Licence:
http://creativecommons.org/licenses/by/4.0
Statistics
Total file downloads (since January 2020). For more information on metrics see the
IRUS guide.