Mouse models of hepatocyte biology - Known unknowns.

Abstract

Mouse models have been fundamental to many significant mechanistic findings that underpin our understanding of the pathophysiology of liver disease. The ability to manipulate genes and pathways allowing us to establish causation, makes them an invaluable tool in liver-related research. Development of novel genetic models has accelerated over recent years with the use of CRISPR/Cas9, permitting faster and more accurate genome editing. However, it has become apparent that genomic modification is not simply deterministic and that potential unintended consequences beyond the intended target locus will only be identified with careful study and validation of the model. In a fascinating new work in this issue of Journal of Hepatology, May et al[1] have performed a detailed examination of such a model and whilst the fine details are important to those in the field of liver homeostasis and regeneration, their findings have broader implications to all who use mouse models in their research.