Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry.

Technical recommendations for analyzing oxylipins by liquid chromatography-mass spectrometry.

Accepted version

Peer-reviewed

Repository URI

https://www.repository.cam.ac.uk/handle/1810/393789

Repository DOI

https://doi.org/10.17863/CAM.123969

Files

Primary Accepted version (175.76 KB)

Type

Authors

Schebb, Nils Helge

https://orcid.org/0000-0003-1299-6629

Kampschulte, Nadja

https://orcid.org/0000-0002-6253-9939

Hagn, Gerhard

https://orcid.org/0000-0001-6782-4117

Plitzko, Kathrin

https://orcid.org/0009-0003-3820-3452

Meckelmann, Sven W

https://orcid.org/0000-0002-0407-7879

Abstract

Several oxylipins are potent lipid mediators that regulate diverse aspects of health and disease and whose quantitative analysis by liquid chromatography-mass spectrometry (LC-MS) presents substantial technical challenges. As members of the lipidomics community, we developed technical recommendations to ensure best practices when quantifying oxylipins by LC-MS.

Keywords

Oxylipins, Chromatography, Liquid, Mass Spectrometry, Humans, Lipidomics, Animals, Liquid Chromatography-Mass Spectrometry

Journal Title

Sci Signal

Journal ISSN

1945-0877
1937-9145

Volume Title

18

Publisher

American Association for the Advancement of Science (AAAS)

Publisher DOI

https://doi.org/10.1126/scisignal.adw1245

Rights and licensing

Attribution 4.0 International

Except where otherwised noted, this item's license is described as Attribution 4.0 International

Sponsorship

Wellcome Trust (220257/Z/20/Z)
MRC (MC_UU_00028/4)

Collections

University of Cambridge Research Outputs (Articles and Conferences)