Genetic tool development in marine protists: emerging model organisms for experimental cell biology


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Authors
Faktorová, Drahomíra  ORCID logo  https://orcid.org/0000-0001-9623-2233
Nisbet, R. Ellen R.  ORCID logo  https://orcid.org/0000-0003-4487-196X
Fernández Robledo, José A. 
Casacuberta, Elena 
Sudek, Lisa 
Abstract

Abstract: Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.

Description
Keywords
Resource, /631/1647/767/722, /631/337, resource
Journal Title
Nature Methods
Conference Name
Journal ISSN
1548-7091
1548-7105
Volume Title
17
Publisher
Nature Publishing Group US
Sponsorship
Gordon and Betty Moore Foundation (Gordon E. and Betty I. Moore Foundation) (GBMF4972 and 4972.01, GBMF4974 and 4974.01, GBMF4983 and 4983.01, GBMF4968 and 4968.01, GBMF4979, GBMF4963 and 4963.01, GBMF4984, GBMF4964, GBMF4976 and 4976.01, GBMF4964, GBMF4965, GBMF4980 and 4980.01, GBMF4986, GBMF4985, GBMF4973 and 4973.01, GBMF4958, GBMF4962.01, GBMF4960, GBMF4957, GBMF4981, GBMF4975 and 4975.01, GBMF4977 and 4977.01, GBMF4961, GBMF4970 and 4970.01)