Genetic tool development in marine protists: emerging model organisms for experimental cell biology

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Faktorová, Drahomíra  ORCID logo
Nisbet, R. Ellen R.  ORCID logo
Fernández Robledo, José A. 
Casacuberta, Elena 
Sudek, Lisa 

Abstract: Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.

Resource, /631/1647/767/722, /631/337, resource
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Nature Methods
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Nature Publishing Group US
Gordon and Betty Moore Foundation (Gordon E. and Betty I. Moore Foundation) (GBMF4972 and 4972.01, GBMF4974 and 4974.01, GBMF4983 and 4983.01, GBMF4968 and 4968.01, GBMF4979, GBMF4963 and 4963.01, GBMF4984, GBMF4964, GBMF4976 and 4976.01, GBMF4964, GBMF4965, GBMF4980 and 4980.01, GBMF4986, GBMF4985, GBMF4973 and 4973.01, GBMF4958, GBMF4962.01, GBMF4960, GBMF4957, GBMF4981, GBMF4975 and 4975.01, GBMF4977 and 4977.01, GBMF4961, GBMF4970 and 4970.01)