Single cell RNA-seq analysis reveals temporally-regulated and quiescence-regulated gene expression in Drosophila larval neuroblasts.


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Authors
Cocanougher, Ben 
Sood, Chhavi 
Yuan, Xin 
Kohn, Andrea B 
Abstract

The mechanisms that generate neural diversity during development remains largely unknown. Here, we use scRNA-seq methodology to discover new features of the Drosophila larval CNS across several key developmental timepoints. We identify multiple progenitor subtypes - both stem cell-like neuroblasts and intermediate progenitors - that change gene expression across larval development, and report on new candidate markers for each class of progenitors. We identify a pool of quiescent neuroblasts in newly hatched larvae and show that they are transcriptionally primed to respond to the insulin signaling pathway to exit from quiescence, including relevant pathway components in the adjacent glial signaling cell type. We identify candidate "temporal transcription factors" (TTFs) that are expressed at different times in progenitor lineages. Our work identifies many cell type specific genes that are candidates for functional roles, and generates new insight into the differentiation trajectory of larval neurons.

Description
Keywords
Research, Neuroblast, Intermediate neural progenitor, Temporal transcription factor, Single cell RNA-sequencing, Quiescence, Insulin signaling
Journal Title
Neural Dev
Conference Name
Journal ISSN
1749-8104
1749-8104
Volume Title
Publisher
Springer Science and Business Media LLC
Sponsorship
Howard Hughes Medical Institute (n/a, n/a)