Bacterial growth, detachment and cell size control on polyethylene terephthalate surfaces.


Type
Article
Change log
Authors
Wang, Liyun 
Fan, Daming 
Chen, Wei 
Terentjev, Eugene M 
Abstract

In medicine and food industry, bacterial colonisation on surfaces is a common cause of infections and severe illnesses. However, the detailed quantitative information about the dynamics and the mechanisms involved in bacterial proliferation on solid substrates is still lacking. In this study we investigated the adhesion and detachment, the individual growth and colonisation, and the cell size control of Escherichia coli (E. coli) MG1655 on polyethylene terephthalate (PET) surfaces. The results show that the bacterial growth curve on PET exhibits the distinct lag and log phases, but the generation time is more than twice longer than in bulk medium. Single cells in the lag phase are more likely to detach than clustered ones in the log phase; clustered bacteria in micro-colonies have stronger adhesive bonds with surfaces and their neighbours with the progressing colonisation. We show that the cell size is under the density-dependent pathway control: when the adherent cells are at low density, the culture medium is responsible for coordinating cell division and cell size; when the clustered cells are at high population density, we demonstrate that the effect of quorum sensing causes the cell size decrease as the cell density on surfaces increases.

Description
Keywords
Bacterial Adhesion, Biofilms, Cell Enlargement, Cell Proliferation, Escherichia coli, Materials Testing, Polyethylene Terephthalates, Surface Properties
Journal Title
Sci Rep
Conference Name
Journal ISSN
2045-2322
2045-2322
Volume Title
5
Publisher
Springer Science and Business Media LLC