Combinatorial discovery of microtopographical landscapes that resist biofilm formation through quorum sensing mediated autolubrication

Abstract

jats:titleAbstract</jats:title> jats:pBio-instructive materials that intrinsically inhibit biofilm formation have significant anti-biofouling potential in industrial and healthcare settings. Since bacterial surface attachment is sensitive to surface topography, we experimentally surveyed 2176 combinatorially generated shapes embossed into polymers using an unbiased screen. This identified microtopographies that, in vitro, reduce colonization by pathogens associated with medical device-related infections by up to 15-fold compared to a flat polymer surface. Machine learning provided design rules, based on generalisable descriptors, for predicting biofilm-resistant microtopographies. On tracking single bacterial cells we observed that the motile behaviour of jats:italicPseudomonas aeruginosa</jats:italic> is markedly different on anti-attachment microtopographies compared with pro-attachment or flat surfaces. Inactivation of Rhl-dependent quorum sensing in jats:italicP. aeruginosa</jats:italic> through deletion of jats:italicrhlI</jats:italic> or jats:italicrhlR</jats:italic> restored biofilm formation on the anti-attachment topographies due to the loss of rhamnolipid biosurfactant production. Exogenous provision of jats:italicN</jats:italic>-butanoyl-homoserine lactone to the jats:italicrhlI</jats:italic> mutant inhibited biofilm formation, as did genetic complementation of the jats:italicrhlI</jats:italic>, jats:italicrhlR</jats:italic> or jats:italicrhlA</jats:italic> mutants. These data are consistent with confinement-induced anti-adhesive rhamnolipid biosurfactant ‘autolubrication’. In a murine foreign body infection model, anti-attachment topographies are refractory to jats:italicP. aeruginosa</jats:italic> colonization. Our findings highlight the potential of simple topographical patterning of implanted medical devices for preventing biofilm associated infections.</jats:p>