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Methylation of the C19MC microRNA locus in the placenta: association with maternal and chilhood body size.

Accepted version
Peer-reviewed

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Article

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Authors

Prats-Puig, Anna 
Xargay-Torrent, Sílvia 
Carreras-Badosa, Gemma 
Mas-Parés, Berta 
Bassols, Judit 

Abstract

OBJECTIVES: To study DNA methylation at the C19MC locus in the placenta and its association with (1) parental body size, (2) transmission of haplotypes for the C19MC rs55765443 SNP, and (3) offspring's body size and/or body composition at birth and in childhood. SUBJECTS AND METHODS: Seventy-two pregnant women-infant pairs and 63 fathers were included in the study. Weight and height of mothers, fathers and newborns were registered during pregnancy or at birth (n = 72). Placental DNA methylation at the C19MC imprinting control region (ICR) was quantified by bisulfite pyrosequencing. Genotyping of the SNP was performed using restriction fragment length polymorphisms. The children's body size and composition were reassessed at age 6 years (n = 32). RESULTS: Lower levels of placental C19MC methylation were associated with increased body size of mother, specifically with higher pregestational and predelivery weights and height of the mother (β from -0.294 to -0.371; R2 from 0.04 to 0.10 and all p < 0.019), and with higher weight, height, waist and hip circumferences, and fat mass of the child (β from -0.428 to -0.552; R2 from 0.33 to 0.56 and all p < 0.009). Parental transmission of the SNP did not correlate with an altered placental methylation status at the C19MC ICR. CONCLUSIONS: Increased maternal size is associated with reduced placental C19MC methylation, which, in turn, relate to larger body size of the child.

Description

Keywords

Adult, Body Size, Child, Chromosomes, Human, Pair 19, DNA Methylation, Fathers, Female, Follow-Up Studies, Humans, Infant, Newborn, Male, MicroRNAs, Mothers, Placenta, Polymorphism, Single Nucleotide, Pregnancy, Young Adult

Journal Title

Int J Obes (Lond)

Conference Name

Journal ISSN

0307-0565
1476-5497

Volume Title

44

Publisher

Springer Science and Business Media LLC

Rights

All rights reserved
Sponsorship
Medical Research Council (G0600717)
Medical Research Council (G0600717/1)
This study was supported by grants from the Ministerio de Ciencia e Innovación, Instituto de 10 Salud Carlos III (ISCIII), Madrid, Spain (PI17/00557 to JB, and PI13/01257 and PI16/01335 to AL-B), projects co-funded by FEDER (Fondo Europeo de Desarrollo Regional). RF and MG acknowledge grant funding from the Fondation pour la Recherche Médicale (Equipes FRM, 13 grant number DEQ31703).