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CD151 regulates expression of FGFR2 in breast cancer cells via PKC-dependent pathways.

Published version
Peer-reviewed

Type

Article

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Authors

Sadej, Rafal 
Lu, Xiaohong 
Turczyk, Lukasz 
Novitskaya, Vera 
Lopez-Clavijo, Andrea F 

Abstract

Expression of the tetraspanin CD151 is frequently upregulated in epithelial malignancies and correlates with poor prognosis. Here, we report that CD151 is involved in regulation of the expression of fibroblast growth factor receptor 2 (FGFR2). Depletion of CD151 in breast cancer cells resulted in an increased level of FGFR2. Accordingly, an inverse correlation between CD151 and FGFR2 was observed in breast cancer tissues. CD151-dependent regulation of the FGFR2 expression relies on post-transcriptional mechanisms involving HuR (also known as ELAVL1), a multifunctional RNA-binding protein, and the assembly of processing bodies (P-bodies). Depletion of CD151 correlated with inhibition of PKC, a well-established downstream target of CD151. Accordingly, the levels of dialcylglycerol species were decreased in CD151-negative cells, and inhibition of PKC resulted in the increased expression of FGFR2. Whereas expression of FGFR2 itself did not correlate with any of the clinicopathological data, we found that FGFR2-/CD151+ patients were more likely to have developed lymph node metastasis. Conversely, FGFR2-/CD151- patients demonstrated better overall survival. These results illustrate functional interdependency between CD151 complexes and FGFR2, and suggest a previously unsuspected role of CD151 in breast tumorigenesis.

Description

Keywords

Breast cancer, CD151, FGFR2, PKC, Tetraspanin, Breast Neoplasms, Carcinogenesis, Cell Line, Tumor, Female, Humans, MCF-7 Cells, Protein Kinase C, Receptor, Fibroblast Growth Factor, Type 2, Signal Transduction, Tetraspanin 24, Transcription, Genetic

Journal Title

J Cell Sci

Conference Name

Journal ISSN

0021-9533
1477-9137

Volume Title

131

Publisher

The Company of Biologists

Rights

Publisher's own licence