Repository logo

Sox17 Controls Emergence and Remodeling of Nestin-Expressing Coronary Vessels.

Accepted version



Change log


González-Hernández, Sara 
Sánchez-Cabo, Fátima  ORCID logo
Méndez-Ferrer, Simón  ORCID logo
Muñoz-Cánoves, Pura 


RATIONALE: The molecular mechanisms underlying the formation of coronary arteries during development and during cardiac neovascularization after injury are poorly understood. However, a detailed description of the relevant signaling pathways and functional TFs (transcription factors) regulating these processes is still incomplete. OBJECTIVE: The goal of this study is to identify novel cardiac transcriptional mechanisms of coronary angiogenesis and vessel remodeling by defining the molecular signatures of coronary vascular endothelial cells during these complex processes. METHODS AND RESULTS: We demonstrate that Nes-gfp and Nes-CreERT2 transgenic mouse lines are novel tools for studying the emergence of coronary endothelium and targeting sprouting coronary vessels (but not ventricular endocardium) during development. Furthermore, we identify Sox17 as a critical TF upregulated during the sprouting and remodeling of coronary vessels, visualized by a specific neural enhancer from the Nestin gene that is strongly induced in developing arterioles. Functionally, genetic-inducible endothelial deletion of Sox17 causes deficient cardiac remodeling of coronary vessels, resulting in improper coronary artery formation. CONCLUSIONS: We demonstrated that Sox17 TF regulates the transcriptional activation of Nestin's enhancer in developing coronary vessels while its genetic deletion leads to inadequate coronary artery formation. These findings identify Sox17 as a critical regulator for the remodeling of coronary vessels in the developing heart.



animals, coronary vessels, developmental biology, endothelium, gene expression regulation, vascular remodeling, Animals, Cell Lineage, Cells, Cultured, Chemokine CXCL12, Coronary Vessels, Endothelial Cells, Gene Expression Regulation, Developmental, HMGB Proteins, Human Umbilical Vein Endothelial Cells, Humans, Mice, Inbred C57BL, Mice, Knockout, Morphogenesis, Neovascularization, Physiologic, Nestin, SOXF Transcription Factors, Transcription, Genetic, Transcriptional Activation, Transcriptome, Vascular Remodeling

Journal Title

Circ Res

Conference Name

Journal ISSN


Volume Title



Ovid Technologies (Wolters Kluwer Health)


All rights reserved
Medical Research Council (MC_PC_12009)
Medical Research Council (MC_PC_17230)