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Capture Hi-C identifies the chromatin interactome of colorectal cancer risk loci.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Jäger, Roland 
Migliorini, Gabriele 
Henrion, Marc 
Kandaswamy, Radhika 
Speedy, Helen E 

Abstract

Multiple regulatory elements distant from their targets on the linear genome can influence the expression of a single gene through chromatin looping. Chromosome conformation capture implemented in Hi-C allows for genome-wide agnostic characterization of chromatin contacts. However, detection of functional enhancer-promoter interactions is precluded by its effective resolution that is determined by both restriction fragmentation and sensitivity of the experiment. Here we develop a capture Hi-C (cHi-C) approach to allow an agnostic characterization of these physical interactions on a genome-wide scale. Single-nucleotide polymorphisms associated with complex diseases often reside within regulatory elements and exert effects through long-range regulation of gene expression. Applying this cHi-C approach to 14 colorectal cancer risk loci allows us to identify key long-range chromatin interactions in cis and trans involving these loci.

Description

Keywords

Base Pairing, Cell Line, Tumor, Chromatin, Chromosomes, Human, Pair 8, Colorectal Neoplasms, Genetic Loci, Genetic Predisposition to Disease, Humans, In Situ Hybridization, Fluorescence, Molecular Sequence Annotation, Nucleotide Motifs, Risk Factors, Statistics as Topic

Journal Title

Nat Commun

Conference Name

Journal ISSN

2041-1723
2041-1723

Volume Title

6

Publisher

Springer Science and Business Media LLC
Sponsorship
Biotechnology and Biological Sciences Research Council (BBS/E/B/000C0405)
Biotechnology and Biological Sciences Research Council (BBS/E/B/000C0404)