This data was collected from a series of experiments performed on three sets of selected lines of the species D. melanogaster, D. mauriatiana and D. simulans selected for resistance to the parasitoid wasp L. boulardi. The data included outlines the number of hemocytes in selected and control lines, the encapsulations rates, the costs of resistance and the ability to resist other parasitoids for each species. Also included are the scripts to produce each figure in the paper.