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An accelerated buoyancy adhesion assay combined with 3-D morphometric analysis for assessing osteoblast adhesion on microgrooved substrata.

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Peer-reviewed

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Article

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Authors

Sobral, JM 
Malheiro, VN 
Clyne, TW 
Harris, J 
Rezk, R 

Abstract

An accelerated negative buoyancy method has been developed to assess cell adhesion strength. This method has been used in conjunction with 3-D morphometric analysis to understand the effects of surface topology on cell response. Aligned micro-grooved surface topographies (with a range of groove depths) were produced on stainless steel 316L substrates by laser ablation. An investigation was carried out on the effect of the micro-grooved surface topography on cell adhesion strength, cell and nucleus volumes, cell phenotypic expression and attachment patterns. Increased hydrophobicity and anisotropic wettability was observed on surfaces with deeper grooves. A reduction was noted in cell volume, projected areas and adhesion sites for deeper grooves, linked to lower cell proliferation and differentiation rates and also to reduced adhesion strength. The results suggest that the centrifugation assay combined with three-dimensional cell morphometric analysis has considerable potential for obtaining improved understanding of the cell/substrate interface.

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Keywords

Cell adhesion, Centrifugation, Laser ablation, Surface topography, Cell Adhesion, Cell Differentiation, Cell Proliferation, Cells, Cultured, Humans, Osteoblasts, Surface Properties

Journal Title

J Mech Behav Biomed Mater

Conference Name

Journal ISSN

1751-6161
1878-0180

Volume Title

60

Publisher

Elsevier BV
Sponsorship
The Royal Society (nf100185)
European Research Council (240446)
Financial support for this work has been provided by the EPSRC (grant GR/R95364/01) and by the ERC (grant No. 240446). The authors are grateful to Dr. Tim Nunney, of Thermo-Fisher Scientific, for carrying out the XPS measurements.