Repository logo

Insulin-like Growth Factor II: An Essential Adult Stem Cell Niche Constituent in Brain and Intestine.

Published version



Change log


Ziegler, Amber N 
Feng, Qiang 
Chidambaram, Shravanthi 
Testai, Jaimie M 
Kumari, Ekta 


Tissue-specific stem cells have unique properties and growth requirements, but a small set of juxtacrine and paracrine signals have been identified that are required across multiple niches. Whereas insulin-like growth factor II (IGF-II) is necessary for prenatal growth, its role in adult stem cell physiology is largely unknown. We show that loss of Igf2 in adult mice resulted in a ∼50% reduction in slowly dividing, label-retaining cells in the two regions of the brain that harbor neural stem cells. Concordantly, induced Igf2 deletion increased newly generated neurons in the olfactory bulb accompanied by hyposmia, and caused impairments in learning and memory and increased anxiety. Induced Igf2 deletion also resulted in rapid loss of stem and progenitor cells in the crypts of Lieberkühn, leading to body-weight loss and lethality and the inability to produce organoids in vitro. These data demonstrate that IGF-II is critical for multiple adult stem cell niches.



IGF-II, crypt of Lieberkühn, intestinal stem cell, neural stem cell, neurogenesis, subgranular zone, subventricular zone, Adult Stem Cells, Animals, Biomarkers, Brain, Cell Differentiation, Immunohistochemistry, Insulin-Like Growth Factor II, Intestines, Mice, Mice, Knockout, Mice, Transgenic, Neurogenesis, Olfactory Bulb, Organ Specificity, Stem Cell Niche

Journal Title

Stem Cell Reports

Conference Name

Journal ISSN


Volume Title



Elsevier BV
Medical Research Council (MC_UU_12012/1)
Medical Research Council (MC_UU_12012/5)
MRC (MC_UU_00014/4)
MRC (MC_UU_00014/5)
Medical Research Council (MC_PC_12012)