NanoDam identifies Homeobrain (ARX) and Scarecrow (NKX2.1) as conserved temporal factors in the Drosophila central brain and visual system

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Tang, Jocelyn LY 
Hakes, Anna E 
Krautz, Robert 
Suzuki, Takumi 

Temporal patterning of neural progenitors is an evolutionarily conserved strategy for generating neuronal diversity. Type II neural stem cells in the Drosophila central brain produce transit-amplifying intermediate neural progenitors (INPs) that exhibit temporal patterning. However, the known temporal factors cannot account for the neuronal diversity in the adult brain. To search for missing factors, we developed NanoDam, enabling rapid genome-wide profiling of endogenously tagged proteins in vivo with a single genetic cross. Mapping the targets of known temporal transcription factors with NanoDam revealed that Homeobrain and Scarecrow (ARX and NKX2.1 orthologues) are also temporal factors. We show that Homeobrain and Scarecrow define middle-aged and late INP temporal windows and play a role in cellular longevity. Strikingly, Homeobrain and Scarecrow have conserved functions as temporal factors in the developing visual system. NanoDam enables rapid cell type specific genome-wide profiling with temporal resolution and is easily adapted for use in higher organisms.

ARX, NKX2.1, NanoDam, neural development, neural stem cells, temporal patterning, Animals, Brain, Cell Lineage, Drosophila, Drosophila Proteins, Drosophila melanogaster, Gene Expression Regulation, Developmental, Neural Stem Cells
Journal Title
Developmental Cell
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Volume Title
Wellcome Trust (092545/Z/10/Z)
Wellcome Trust (103792/Z/14/Z)
Royal Society (RP150061)
Wellcome Trust (089616/Z/09/Z)
Wellcome Trust (092096/Z/10/Z)
Cancer Research Uk (None)
Wellcome Trust (102454/Z/13/Z)
Wellcome Trust (203798/Z/16/Z)