The subcellular organisation of Saccharomyces cerevisiae.

Change log
Nightingale, Daniel Jh 
Geladaki, Aikaterini 
Breckels, Lisa M 
Oliver, Stephen G 
Lilley, Kathryn S 

Subcellular protein localisation is essential for the mechanisms that govern cellular homeostasis. The ability to understand processes leading to this phenomenon will therefore enhance our understanding of cellular function. Here we review recent developments in this field with regard to mass spectrometry, fluorescence microscopy and computational prediction methods. We highlight relative strengths and limitations of current methodologies focussing particularly on studies in the yeast Saccharomyces cerevisiae. We further present the first cell-wide spatial proteome map of S. cerevisiae, generated using hyperLOPIT, a mass spectrometry-based protein correlation profiling technique. We compare protein subcellular localisation assignments from this map, with two published fluorescence microscopy studies and show that confidence in localisation assignment is attained using multiple orthogonal methods that provide complementary data.

Mass Spectrometry, Microscopy, Fluorescence, Proteomics, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Journal Title
Curr Opin Chem Biol
Conference Name
Journal ISSN
Volume Title
Elsevier BV
Wellcome Trust (108441/Z/15/Z)
Biotechnology and Biological Sciences Research Council (BB/N023129/1)
Biotechnology and Biological Sciences Research Council (BB/K00137X/1)
Wellcome Trust (110170/Z/15/Z)
Biotechnology and Biological Sciences Research Council (BB/L002817/1)
BBSRC and Wellcome Trust