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Synthesis and biophysical analysis of modified thymine-containing DNA oligonucleotides.

Accepted version
Peer-reviewed

Repository URI

https://www.repository.cam.ac.uk/handle/1810/263112

Repository DOI

https://doi.org/10.17863/CAM.8415
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Accepted version (6.81 MB)

Type

Article

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Authors

Balasubramanian, Shankar  ORCID logo  https://orcid.org/0000-0002-0281-5815

Abstract

We report the synthesis of a 5-formyl-2'-deoxyuridine (5fU) phosphoramidite and the preparation of oligonucleotides comprising all known, naturally observed eukaryotic thymidine modifications. Biophysical characterization of the synthetic oligonucleotides indicates that 5fU, but not the other T-derivatives, can alter DNA structures.

Description

Keywords

DNA, Deoxyuridine, Oligonucleotides, Organophosphorus Compounds, Thymine

Journal Title

Chemical Communications

Conference Name

Journal ISSN

1359-7345
1364-548X

Volume Title

53

Publisher

Royal Society of Chemistry

Publisher DOI

https://doi.org/10.1039/c6cc08670e

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Sponsorship
Wellcome Trust (099232/Z/12/Z)
Cancer Research UK (18618)
This work was supported by the following grants: the Wellcome Trust (099232/Z/12/Z), Cancer Research UK (C14303/A17197), ERC Advanced grant (PM), Herchel Smith Funding and A*STAR (ZL).

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Scholarly Works - Chemistry
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