Synthesis and biophysical analysis of modified thymine-containing DNA oligonucleotides.

Accepted version
Repository URI
https://www.repository.cam.ac.uk/handle/1810/263112
Repository DOI
https://doi.org/10.17863/CAM.8415
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Accepted version (6.81 MB)
Type
Article
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Authors
Kawasaki, F 
Murat, P 
Li, Z 
Santner, T 
Balasubramanian, Shankar  ORCID logo  https://orcid.org/0000-0002-0281-5815
Abstract

We report the synthesis of a 5-formyl-2'-deoxyuridine (5fU) phosphoramidite and the preparation of oligonucleotides comprising all known, naturally observed eukaryotic thymidine modifications. Biophysical characterization of the synthetic oligonucleotides indicates that 5fU, but not the other T-derivatives, can alter DNA structures.

Description
Keywords
DNA, Deoxyuridine, Oligonucleotides, Organophosphorus Compounds, Thymine
Journal Title
Chemical Communications
Conference Name
Journal ISSN
1359-7345
1364-548X
Volume Title
53
Publisher
Royal Society of Chemistry
Publisher DOI
https://doi.org/10.1039/c6cc08670e
Rights
http://www.rioxx.net/licenses/all-rights-reserved
Sponsorship
Wellcome Trust (099232/Z/12/Z)
This work was supported by the following grants: the Wellcome Trust (099232/Z/12/Z), Cancer Research UK (C14303/A17197), ERC Advanced grant (PM), Herchel Smith Funding and A*STAR (ZL).
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Scholarly Works - Chemistry
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