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A Platform for Site‐Specific DNA‐Antibody Bioconjugation by Using Benzoylacrylic‐Labelled Oligonucleotides

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Konč, Juraj 
Brown, Libby 
Whiten, Daniel R. 
Zuo, Yukun 


Abstract: Many bioconjugation strategies for DNA oligonucleotides and antibodies suffer limitations, such as site‐specificity, stoichiometry and hydrolytic instability of the conjugates, which makes them unsuitable for biological applications. Here, we report a new platform for the preparation of DNA‐antibody bioconjugates with a simple benzoylacrylic acid pentafluorophenyl ester reagent. Benzoylacrylic‐labelled oligonucleotides prepared with this reagent can be site‐specifically conjugated to a range of proteins and antibodies through accessible cysteine residues. The homogeneity of the prepared DNA‐antibody bioconjugates was confirmed by a new LC‐MS protocol and the bioconjugate probes were used in fluorescence or super‐resolution microscopy cell imaging experiments. This work demonstrates the versatility and robustness of our bioconjugation protocol that gives site‐specific, well‐defined and plasma‐stable DNA‐antibody bioconjugates for biological applications.



Research Article, Research Articles, antibodies, bioconjugation, imaging, mass spectrometry, nucleic acids

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Angewandte Chemie International Edition

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biotechnology and biological sciences research council (iCASE)
h2020 european research council (676832)
royal society (RP150066, URF\R\180019)
fundação para a ciência e a tecnologia (CEECIND/00453/2018)