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vLUME: 3D virtual reality for single-molecule localization microscopy.

Accepted version
Peer-reviewed

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Type

Article

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Authors

Spark, Alexander 
Kitching, Alexandre 
Esteban-Ferrer, Daniel  ORCID logo  https://orcid.org/0000-0001-9542-1147
Handa, Anoushka 
Carr, Alexander R 

Abstract

vLUME is a virtual reality software package designed to render large three-dimensional single-molecule localization microscopy datasets. vLUME features include visualization, segmentation, bespoke analysis of complex local geometries and exporting features. vLUME can perform complex analysis on real three-dimensional biological samples that would otherwise be impossible by using regular flat-screen visualization programs.

Description

Keywords

Algorithms, Animals, COS Cells, Caulobacter crescentus, Cell Line, Cell Membrane, Chlorocebus aethiops, Clathrin, Humans, Image Processing, Computer-Assisted, Jurkat Cells, Microtubules, Nuclear Pore, Single Molecule Imaging, Software, Virtual Reality

Journal Title

Nat Methods

Conference Name

Journal ISSN

1548-7091
1548-7105

Volume Title

17

Publisher

Springer Science and Business Media LLC

Rights

All rights reserved
Sponsorship
Engineering and Physical Sciences Research Council (EP/M003663/1)
The Royal Society (uf120277)
Royal Society (URF\R\180029)
Royal Society (RGF\EA\181021)
Engineering and Physical Sciences Research Council (EP/N509620/1)
Wellcome Trust (via University of Oxford) (207547/Z/17/Z)
RS URF, EPSRC Doctoral training awards, EPSRC follow on fund
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