DNA-Tile Structures Induce Ionic Currents through Lipid Membranes.
Meijering, Anna EC
Keyser, Ulrich F
American Chemical Society (ACS)
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Göpfrich, K., Zettl, T., Meijering, A. E., Hernández-Ainsa, S., Kocabey, S., Liedl, T., & Keyser, U. F. (2015). DNA-Tile Structures Induce Ionic Currents through Lipid Membranes.. Nano Lett, 15 3134-3138. https://doi.org/10.1021/acs.nanolett.5b00189
Self-assembled DNA nanostructures have been used to create man-made transmembrane channels in lipid bilayers. Here, we present a DNA-tile structure with a nominal subnanometer channel and cholesterol-tags for membrane anchoring. With an outer diameter of 5 nm and a molecular weight of 45 kDa, the dimensions of our synthetic nanostructure are comparable to biological ion channels. Because of its simple design, the structure self-assembles within a minute, making its creation scalable for applications in biology. Ionic current recordings demonstrate that the tile structures enable ion conduction through lipid bilayers and show gating and voltage-switching behavior. By demonstrating the design of DNA-based membrane channels with openings much smaller than that of the archetypical six-helix bundle, our work showcases their versatility inspired by the rich diversity of natural membrane components.
DNA nanotechnology, artificial ion channels, DNA-tiles, single-molecule, lipid bilayer, self-assembly
K.G. acknowledges funding from the Winton Programme for the Physics of Sustainability, Gates Cambridge, and the Oppenheimer Ph.D. studentship. T.Z. and A.E.C.M. acknowledge funding from the ERASMUS programme and S.H.A. from a Herchel Smith postdoctoral fellowship. S.K. and T.L. acknowledge funding from the DFG Nanosystems Initiative Munich. U.F.K. acknowledges funding from an ERC starting grant Passmembrane 261101 and Oxford Nanopore Technologies.
European Research Council (261101)
External DOI: https://doi.org/10.1021/acs.nanolett.5b00189
This record's URL: https://www.repository.cam.ac.uk/handle/1810/259995