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dc.contributor.authorGöpfrich, Kerstin
dc.contributor.authorZettl, Thomas
dc.contributor.authorMeijering, Anna EC
dc.contributor.authorHernández-Ainsa, Silvia
dc.contributor.authorKocabey, Samet
dc.contributor.authorLiedl, Tim
dc.contributor.authorKeyser, Ulrich F
dc.date.accessioned2016-09-07T11:34:03Z
dc.date.available2016-09-07T11:34:03Z
dc.date.issued2015-05-13
dc.identifier.issn1530-6984
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/259995
dc.description.abstractSelf-assembled DNA nanostructures have been used to create man-made transmembrane channels in lipid bilayers. Here, we present a DNA-tile structure with a nominal subnanometer channel and cholesterol-tags for membrane anchoring. With an outer diameter of 5 nm and a molecular weight of 45 kDa, the dimensions of our synthetic nanostructure are comparable to biological ion channels. Because of its simple design, the structure self-assembles within a minute, making its creation scalable for applications in biology. Ionic current recordings demonstrate that the tile structures enable ion conduction through lipid bilayers and show gating and voltage-switching behavior. By demonstrating the design of DNA-based membrane channels with openings much smaller than that of the archetypical six-helix bundle, our work showcases their versatility inspired by the rich diversity of natural membrane components.
dc.description.sponsorshipK.G. acknowledges funding from the Winton Programme for the Physics of Sustainability, Gates Cambridge, and the Oppenheimer Ph.D. studentship. T.Z. and A.E.C.M. acknowledge funding from the ERASMUS programme and S.H.A. from a Herchel Smith postdoctoral fellowship. S.K. and T.L. acknowledge funding from the DFG Nanosystems Initiative Munich. U.F.K. acknowledges funding from an ERC starting grant Passmembrane 261101 and Oxford Nanopore Technologies.
dc.languageEnglish
dc.language.isoen
dc.publisherAmerican Chemical Society (ACS)
dc.subjectDNA nanotechnology
dc.subjectartificial ion channels
dc.subjectDNA-tiles
dc.subjectsingle-molecule
dc.subjectlipid bilayer
dc.subjectself-assembly
dc.titleDNA-Tile Structures Induce Ionic Currents through Lipid Membranes.
dc.typeArticle
dc.description.versionThis is the author accepted manuscript. The final version is available from ACS Publications via http://dx.doi.org/10.1021/acs.nanolett.5b00189
prism.endingPage3138
prism.publicationDate2015
prism.publicationNameNano Lett
prism.startingPage3134
prism.volume15
dc.identifier.doi10.17863/CAM.4222
dcterms.dateAccepted2015-03-27
rioxxterms.versionofrecord10.1021/acs.nanolett.5b00189
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2015-03-27
dc.contributor.orcidKeyser, Ulrich [0000-0003-3188-5414]
dc.identifier.eissn1530-6992
rioxxterms.typeJournal Article/Review
pubs.funder-project-idEuropean Research Council (261101)
cam.issuedOnline2015-04-08


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