Single molecule based SNP detection using designed DNA carriers and solid-state nanopores
Accepted version
Peer-reviewed
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Abstract
Single nucleotide polymorphisms (SNPs) play a crucial role as molecular markers in medical and diagnostic application. We demonstrate a nanopore based method for SNP detection at the single molecule level. Designed DNA carriers are used to distinguish DNA strands containing only one single base difference and follow strand displacement kinetics.
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Keywords
Biosensing Techniques, DNA, DNA Probes, Kinetics, Models, Molecular, Nanopores, Nanotechnology, Nucleic Acid Conformation, Polymorphism, Single Nucleotide
Journal Title
Chemical Communications
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Journal ISSN
1359-7345
1364-548X
1364-548X
Volume Title
53
Publisher
Royal Society of Chemistry
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Sponsorship
European Research Council (647144)
J. Kong acknowledges funding from Chinese Scholarship Council and Cambridge Trust. J. Zhu and UFK acknowledge support from an ERC Consolidator grant (DesignerPores 647144).