Photoacoustic imaging using genetically encoded reporters: a review
Publication Date
2017-07Journal Title
Journal of Biomedical Optics
ISSN
1083-3668
Publisher
S P I E - International Society for Optical Engineering
Volume
22
Issue
7
Number
070901
Language
English
Type
Article
This Version
VoR
Metadata
Show full item recordCitation
Brunker, J., Yao, J., Laufer, J., & Bohndiek, S. (2017). Photoacoustic imaging using genetically encoded reporters: a review. Journal of Biomedical Optics, 22 (7. 070901)https://doi.org/10.1117/1.JBO.22.7.070901
Abstract
Genetically encoded contrast in photoacoustic imaging (PAI) is complementary to the intrinsic contrast provided by endogenous absorbing chromophores such as hemoglobin. The use of reporter genes expressing absorbing proteins opens the possibility of visualizing dynamic cellular and molecular processes. This is an enticing prospect but brings with it challenges and limitations associated with generating and detecting different types of reporters. The purpose of this review is to compare existing PAI reporters and signal detection strategies, thereby offering a practical guide, particularly for the nonbiologist, to choosing the most appropriate reporter for maximum sensitivity in the biological and technological system of interest.
Keywords
genetic reporters, in vivo, photoacoustics
Sponsorship
J.B. and S.E.B. are supported by the EPSRCCRUK Cancer Imaging Centre in Cambridge and Manchester (No. C197/A16465); CRUK (Nos. C14303/A17197 and C47594/A16267); and the European Union’s Seventh Framework Programme (No. FP7/2007-2013) under Grant Agreement No. FP7-PEOPLE-2013-CIG-630729. J.Y. is partly supported by Duke MEDx Basic Research Grant. J.L. acknowledges the support of ERC Starting Grant No. 281356.
Funder references
Cancer Research UK (16465)
Cancer Research UK (C14303_do not transfer)
European Commission (630729)
Embargo Lift Date
2100-01-01
Identifiers
External DOI: https://doi.org/10.1117/1.JBO.22.7.070901
This record's URL: https://www.repository.cam.ac.uk/handle/1810/265106
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