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Assessing sub-cellular resolution in spatial proteomics experiments.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Breckels, Lisa M 
Lilley, Kathryn S 

Abstract

The sub-cellular localisation of a protein is vital in defining its function, and a protein's mis-localisation is known to lead to adverse effect. As a result, numerous experimental techniques and datasets have been published, with the aim of deciphering the localisation of proteins at various scales and resolutions, including high profile mass spectrometry-based efforts. Here, we present a meta-analysis assessing and comparing the sub-cellular resolution of 29 such mass spectrometry-based spatial proteomics experiments using a newly developed tool termed QSep. Our goal is to provide a simple quantitative report of how well spatial proteomics resolve the sub-cellular niches they describe to inform and guide developers and users of such methods.

Description

Keywords

Animals, Cellular Structures, Humans, Mass Spectrometry, Proteins, Proteomics

Journal Title

Curr Opin Chem Biol

Conference Name

Journal ISSN

1367-5931
1879-0402

Volume Title

48

Publisher

Elsevier BV
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/L018497/1)
Wellcome Trust (108441/Z/15/Z)
Wellcome Trust (110170/Z/15/Z)
Biotechnology and Biological Sciences Research Council (BB/K00137X/1)
Biotechnology and Biological Sciences Research Council (BB/N023129/1)
Biotechnology and Biological Sciences Research Council (BB/L002817/1)
This work was supported by a BBSRC Strategic Longer and Larger grant (Award BB/L002817/1), a Wellcome Trust Technology Development Grant (Grant number 108441/Z/15/Z) and a BBSRC Tools and resources development grant (Award BB/N023129/1).