Assessing sub-cellular resolution in spatial proteomics experiments.
Breckels, Lisa M
Lilley, Kathryn S
Curr Opin Chem Biol
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Gatto, L., Breckels, L. M., & Lilley, K. S. (2019). Assessing sub-cellular resolution in spatial proteomics experiments.. Curr Opin Chem Biol, 48 123-149. https://doi.org/10.1016/j.cbpa.2018.11.015
The sub-cellular localisation of a protein is vital in defining its function, and a protein's mis-localisation is known to lead to adverse effect. As a result, numerous experimental techniques and datasets have been published, with the aim of deciphering the localisation of proteins at various scales and resolutions, including high profile mass spectrometry-based efforts. Here, we present a meta-analysis assessing and comparing the sub-cellular resolution of 29 such mass spectrometry-based spatial proteomics experiments using a newly developed tool termed QSep. Our goal is to provide a simple quantitative report of how well spatial proteomics resolve the sub-cellular niches they describe to inform and guide developers and users of such methods.
Cellular Structures, Animals, Humans, Proteins, Proteomics, Mass Spectrometry
This work was supported by a BBSRC Strategic Longer and Larger grant (Award BB/L002817/1), a Wellcome Trust Technology Development Grant (Grant number 108441/Z/15/Z) and a BBSRC Tools and resources development grant (Award BB/N023129/1).
Biotechnology and Biological Sciences Research Council (BB/L018497/1)
Wellcome Trust (108441/Z/15/Z)
Wellcome Trust (110170/Z/15/Z)
Biotechnology and Biological Sciences Research Council (BB/K00137X/1)
Biotechnology and Biological Sciences Research Council (BB/N023129/1)
Biotechnology and Biological Sciences Research Council (BB/L002817/1)
External DOI: https://doi.org/10.1016/j.cbpa.2018.11.015
This record's URL: https://www.repository.cam.ac.uk/handle/1810/287483
Attribution 4.0 International
Licence URL: https://creativecommons.org/licenses/by/4.0/