Assessing sub-cellular resolution in spatial proteomics experiments.
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Peer-reviewed
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The sub-cellular localisation of a protein is vital in defining its function, and a protein's mis-localisation is known to lead to adverse effect. As a result, numerous experimental techniques and datasets have been published, with the aim of deciphering the localisation of proteins at various scales and resolutions, including high profile mass spectrometry-based efforts. Here, we present a meta-analysis assessing and comparing the sub-cellular resolution of 29 such mass spectrometry-based spatial proteomics experiments using a newly developed tool termed QSep. Our goal is to provide a simple quantitative report of how well spatial proteomics resolve the sub-cellular niches they describe to inform and guide developers and users of such methods.
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1879-0402
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Wellcome Trust (108441/Z/15/Z)
Wellcome Trust (110170/Z/15/Z)
Biotechnology and Biological Sciences Research Council (BB/K00137X/1)
Biotechnology and Biological Sciences Research Council (BB/N023129/1)
Biotechnology and Biological Sciences Research Council (BB/L002817/1)